Melanoma Cell Lines: VGP-Like

VGP-Like melanoma cell lines.

Cell line

Sex/Age

AJCC stage/
pathology

Level/
thickness (mm)

Mitotic rate

Specimen site

Primary reference

Isolation
date

WM75

M 48

Stage 3

SSM

RGP/VGP

IV/6.25

3.8

L back/

chest

(5-8)

07/17/79

WM98-1

M 50

Stage 2

SSM

RGP/VGP

IV/5.40

6.5

chest

(1,7,8)

11/30/79

WM115

F 55

Stage n.a.

SSM

RGP/VGP

III/2.24

10.3

groin

R leg

(3,5-8)

04/30/80

WM278

F 62

Stage 2

NM

VGP only

IV/3.7

n.a .

n.a .

(1-3,8)

11/11/81

WM740V

F 34

Stage 2

SSM

RGP/VGP

III/3.24

n.a .

chest

(8)

n.a .

WM793B

M 37

Stage 1

SSM

RGP/VGP

II/0.55

n.a .

sternum

(1,3,5,7,8)

01/07/83

WM853-2

M 70

Stage 2

SSM

RGP/VGP

IV/3.75

n.a .

back

(8)

05/25/83

WM902B

n.a .

Stage n.a.

SSM

VGP

n.a .

n.a .

n.a .

(1,5,7)

11/14/83

WM983A

M 54

Stage n.a.

URGP

RGP/VGP

IV/25.0

n.a .

abdomen

(7,8)

08/13/84

WM1341D

M 85

Stage n.a.

LMM

RGP/VGP

IV/3.70

n.a .

n.a .

n.a .

07/28/86

WM1361A

M 50

Stage x

SSM

RGP/VGP

IV/1.22

2.2

forearm

(7,8)

08/27/86

WM3268V

F 70

Stage 3

n.a .

n.a .

leg

n.a .

n.a .

References:

1. Herlyn , M., Thurin, J., Balaban, G., Bennicelli, J. L., Herlyn, D., Elder, D.,E.,  Clark, W.H., and Koprowski, H. Characteristics of cultured human malanocytes isolated from different stages of tumor progression. Cancer Res. 45: 5670, 1985.

2. Balaban, G., Herlyn, M., Guerry, D., IV, Bartolo, R., Koprowski, H., Clark, W.H., and Nowell, P.C. Cytogenics of human malignant melanoma and premalignant lesions. Cancer Genet. Cytogenet. 11: 428, 1984.

3. Satyamoorthy, K., DeJesus, E., Linnenbach, A.,J., Kraj, B. Kornreich, D.L., Rendel, S., Elder, D., and Herlyn, M. Melanoma cell lines from different stages of progression and their biological and molecular analyses. Melanoma Res. 7: S35, 1997.

4. Easty, D. J., Herlyn, M. and Bennett, D.C. Abnormal protein tyrosine kinase gene expression during melanoma progression and metastasis. Int. J. Cancer 60: 129. 1995.

5. Rodeck, U., Herlyn, M., Menssen, H.D., Furlanetto, R.W., Koprowski, H.: Metastatic but not primary melanoma cell lines grow in vitro independently of exogenous growth factors. Int J Cancer 40: 687, 1987.

6. Herlyn, M., Balaban, G., Bennicelli, J., Guerry, D.t., Halaban, R., Herlyn, D., Elder, D.E., Maul, G.G., Steplewski, Z., Nowell, P.C., et al.: Primary melanoma cells of the vertical growth phase: similarities to metastatic cells. J Natl Cancer Inst 74:283-289, 1985.

7. Kath, R., Rodeck, U., Parmiter, A., Jambrosic, J., Herlyn, M.: Growth factor independence in vitro of primary melanoma cells from advanced but not early or intermediate lesions. Cancer Therapy and Control 1:179-191, 1990.

8. Gyorfi, T. and Herlyn, M. Melanocyte and melanoma cell lines. In: R. J. Hay, J. G. Park and A. Gazdar (eds.), Atlas of Human Tumor Cell Lines. P. 413, Academic Press, Inc., 1994.

Genetic abnormalities in VGP-like melanoma cell lines.

Cell line

BRAF

n-ras

k-ras

P53

WM75

V599E

WT

WT

WT

WM98-1

NT

NT

NT

NT

WM115

V599D

WT

WT

WT

WM278

V599E

WT

WT

WT

WM740V

NT

NT

NT

NT

WM793B

V599E

WT

WT

NT

WM853-2

NT

NT

NT

NT

WM902B

V599E

WT

WT

NT

WM983A

V599E

WT

WT

NT

WM1341D

V599R

WT

NT

NT

WM1361A

WT

61

WT

NT

WM3268V

V599E