Components |
|
HES |
||||
[Cfinal] |
V = 250 ml |
V = 100 ml |
||||
DMEM/F-12 (Invitrogen Cat. No. 11330-032) |
|
80% |
200 ml |
80 ml |
||
KnockOut™ SR (Invitrogen Cat. No. 10828-028) |
20% |
50 ml |
20 ml |
|||
L-glutamine (Invitrogen Cat. No. 21051-024; 0.146 g/10 ml DPBS, add 7 μl β-mercaptoethanol prior using) |
1.25 ml |
1 ml |
||||
MEM Non-Essential Amino Acids Solution 10 mM (100X), (Invitrogen Cat. No. 11140-050) |
1% |
2.5 ml |
1 ml |
|||
bFGF (2 μg/ml) |
4 ng/ml |
0.5 ml |
0.2 ml |
|||
Sterilized by using 0.22 μm CA filter |
||||||
Components |
HESCM4 |
|||||
[Cfinal] |
V = 250 ml |
V = 100 ml |
||||
HESCM |
70% |
175 ml |
70 ml |
|||
HES |
30% |
75 ml |
30 ml |
|||
Cryo-preservative Medium for Melanoma Stem Cells (2X) |
||||||
KnockOut™ SR (Invitrogen Cat. No. 10828-028) |
60% |
|||||
DMSO (Sigma Cat. No. D2650) |
20% |
|||||
HES |
20% |
|||||
To freeze Melanoma Stem Cells: 1V of 2X Cryo-preservative medium + 1V of hESCM4 |
||||||