Study Details Regulation of Vital Tumor Suppressor Gene p53
Study Details Regulation of Vital Tumor Suppressor Gene p53
So vital is the p53 tumor suppressor gene in controlling cancer that its dysfunction is linked to more than half of human cancers. At the same time, the gene’s capacity for shutting down cell growth, even causing cells to commit suicide if necessary, is so absolute that it must be tightly regulated to maintain the optimal balance between protecting against cancer and permitting normal growth.
Now, a study by scientists at The Wistar Institute reveals new levels of subtlety in the body’s management of this all-important tumor suppressor gene and the protein it produces. The experiments show that, while the addition of a specific molecule at a particular site on the p53 protein prevents it from acting, the addition of a second copy of the same molecule at the same site reverses the effect, sending p53 into action. Further, removal of the second copy returns the protein to its repressed state.
In addition to the implications for understanding the activity of the p53 gene, the findings also outline an important new cycle of gene-regulating modifications involving the addition and removal of the molecules, called methyl groups, that may be widespread in the genome. A report on the study appears in the September 6 issue of Nature.
“The p53 tumor suppressor is extremely potent in halting cell growth,” says Shelley L. Berger, Ph.D., the Hilary Koprowski Professor at The Wistar Institute and senior author on the study. “So, as critical as p53 is in protecting against the unchecked growth of cancer, you don’t want it constantly on. If it were always on, your cells wouldn’t be able to grow normally. Yet it needs to be constantly on call for activation against cancer and other aberrant cellular developments. Our study shows one way that the cell, working at one particular location on the p53 protein, maintains a nuanced but firm control over the gene’s activity.”
Responsible for tumor suppression throughout the body, the p53 gene is mutated or otherwise disabled in a majority of human cancers. When working properly, the protein produced by the p53 gene acts by binding to DNA to activate other genes that direct cells with damaged DNA to cease dividing until the damage can be repaired. Cells with such damage include cancer cells, since all cancers track to genetic flaws of one kind or another, whether inherited or acquired. If repairs cannot be made, p53 commands the cells with damaged DNA to self-destruct so they are no longer a danger to the body.
This powerful ability of p53 to shut down cell division and induce cell death points to why fine-tuned regulatory mechanisms such as the one outlined in the new study are crucial for cellular survival.
In a previous study published in Nature in November 2006, Berger and her colleagues showed that the addition of a single methyl group – a tiny molecule consisting of one carbon and three hydrogen atoms – at a specific site on the p53 protein was sufficient to repress its activity. In the current study, the researchers found that the addition of a second methyl group at the same site reversed the effect. With the pair of methyl groups in place, the site is able to attract and bind a molecule called 53bp1, itself required for the p53 protein to bind to DNA to launch the genes responsible for carrying out its tumor-suppressing mission. With one methyl group in place, the site is said to be monomethylated; with two in place, it is dimethylated.
“An important finding from our study is that the dimethylation mark is the required recognition site for 53bp1 on the p53 protein,” says Jing Huang, Ph.D., lead author on the Nature study. “If you remove that mark, 53bp1 cannot associate with the p53 protein, and p53’s activity will be reduced.”
In addition to lead author Huang and senior author Berger, the other Wistar-based co-authors are Min Gyu Lee, Jean A. Dorsey, and professor Ramin Shiekhattar. The remaining co-authors are Roopsha Sengupta, Mario Richter, Susanne Opravil, and Thomas Jenuwein at The Vienna Biocenter in Austria, and Alexsandra B. Espejo and Mark T. Bedford at the M.D. Anderson Cancer Center in Texas.
Funding for the research was provided to the Berger laboratory by the National Cancer Institute of the National Institutes of Health and the Commonwealth Universal Research Enhancement Program of the Pennsylvania Department of Health. Support for the Jenuwein laboratory was provided by Boehringer Ingelheim, the European Union, and the Austrian Ministry of Education, Science, and Culture. Additional support came from an American Association for Cancer Research-Pennsylvania Department of Health Fellows grant and a Lymphoma Society Special Fellow grant to Huang and a Welch Foundation grant to Bedford.
The Wistar Institute is an international leader in biomedical research, with special expertise in cancer research and vaccine development. Founded in 1892 as the first independent nonprofit biomedical research institute in the country, Wistar has long held the prestigious Cancer Center designation from the National Cancer Institute. Discoveries at Wistar have led to the creation of the rubella vaccine that eradicated the disease in the U.S., rabies vaccines used worldwide, and a new rotavirus vaccine approved in 2006. Wistar scientists have also identified many cancer genes and developed monoclonal antibodies and other important research tools. Today, Wistar is home to eminent melanoma researchers and pioneering scientists working on experimental vaccines against influenza, HIV, and other diseases threatening global health. The Institute works actively to transfer its inventions to the commercial sector to ensure that research advances move from the laboratory to the clinic as quickly as possible. The Wistar Institute: Today’s Discoveries – Tomorrow’s Cures. On the web at www.wistar.org.