Microscopes

Widefield Microscopes
                             
Nikon E600 Upright Microscope  
Location: Room B78

Lenses
2X, 4X, 10X, 20X, 40X, 60X and 100X oil
Illumination options
Brightfield, Darkfield, Fluorescence, Polarization
Fluorescence Filter Cubes
DAPI, FITC, TRITC, CFP, YFP, (triple cube available for viewing only)

Image Capture
Spot RT Slider digital camera w/ ImagePro Plus software for fluorescence monochrome capture - 1600 x 1200 pixel resolution.
Nikon DXM 1200F digital camera w/ Nikon ACT1 software for color capture – 
Variable pixel resolution, 1280 x 1024 pixels standard

Workstation

Pentium 4 Dell PC with ImagePro v.4.5, 48X CD reader/writer, Zip 250 drive, front USB port

 

Leica DMRE Upright Microscope (For Fluorescence)
Location: Room B80

Lenses
2.5X, 10X, 20X, 40X and 100X oil
Illumination options
Brightfield, Fluorescence, 
Fluorescence Filter Cubes
DAPI, GFP/Aqua, HQ FITC, Piston GFP, HQ R, CY5.5, G/R (for viewing only), GFP/DsRed FRET
Image Capture
Hammamatsu ORCA digital camera w/ ImagePro Plus software for fluorescence monochrome capture – 1344 x 1024 or 672 x 512 pixel resolution.

Workstation

Pentium 4 Dell PC with ImagePro v.6.2, 48X CD reader/writer, Zip 250 drive, front USB port

 

Zeiss Axioplan Upright Microscope (for Brightfield)
Location: Room B80

Lenses: 10X, 20X, 40X and 100X oil
Illumination options: Brightfield, 
Image Capture: 
Nikon DXM 1200F digital camera w/ Nikon ACT1 software for color capture – 
Variable pixel resolution, 1280 x 1024 pixels standard

Workstation

Pentium 4 Dell PC with 48X CD reader/writer, Zip 250 drive, front USB port (share with SMZ800 steromicroscope)

 

Nikon TE300 Inverted Microscope (Live-Cell Time-Lapse System)
Location: Room B78

Lenses
4X, 10X, 20X, 40X (all Long Working Distance)
2X (non-phase), 60X (non-phase, close working distance) and 100X oil (non-phase) available
Illumination options
Brightfield, Phase, Oblique, Fluorescence
Fluorescence Filter Cubes
DAPI, FITC, TRITC, CFP, YFP, (triple cube available for viewing only)
Fluorescence Filter Wheel:
DAPI, Pacific Blue, CFP, GFP, TRITC, CY5

Motorized XY Stage
Multiple inserts for well plates, 35mm dishes, standard glass slides. Positions can be saved for return sessions.

Environmental Chamber
Temperature and CO2 control surrounding entire microscope
Image Capture
  -   Q-Imaging Retiga EX digital camera, high sensitivity monochrome camera 1360 x 1036 pixel resolution
-   Single-shot color imaging through front camera port with Nikon D200 digital SLR camera

Workstation
Dell Core 2 Duo with ImagePro v.6.2, CD reader/writer, DVD reader/writer, USB and Firewire connections,  Dual 19” Flat Screens

Additional
Z-axis focusing controller with encoder, Automatic fluorescence shutter

Time-Lapse Capabilities
6D imaging (X, Y, Z, time, wavelength, positions)

Nikon TE2000 Inverted Microscope 
Location: Room B78

Lenses
4X, 10X, 20X, 40X (all Long Working Distance)
2X (non-phase), 60X (non-phase, close working distance) and 100X oil (non-phase) available
Illumination options
Brightfield, Phase, Oblique, Fluorescence
Fluorescence Filter Cubes
DAPI, CFP, FITC, TRITC, Texas Red
Image Capture
-   Q-Imaging Retiga EX digital camera, high sensitivity monochrome camera 
1360 x 1036 pixel resolution
- Nikon DXM 1200F digital camera w/ Nikon ACT1 software for color capture –
Variable pixel resolution, 1280 x 1024 pixels standard
-   Single-shot color imaging available through front camera port with Nikon D200 digital SLR camera

Workstation

Pentium 4 Dell PC with ImagePro v.6.2, CD reader/writer, DVD reader/writer, USB and Firewire connections, 
 

Nikon SMZ800 Stereo Microscope
Location: Room B80

Magnification Range: 1X objective with 1X – 6.3X variable zoom
Illumination options: Brightfield, Darkfield, Polarization, Oblique Illumination, Episcopic Lighting, Fiber optic light tubes
Fluorescence: GFP possible with Illumatool 9900 system
Image Capture: - Nikon DXM 1200F digital camera w/ Nikon ACT1 software for color capture – 
Variable pixel resolution, 1280 x 1024 pixels standard

Workstation

Pentium 4 Dell PC with 48X CD reader/writer, Zip 250 drive, front USB port (share with Zeiss Axioplan back-up upright microscope)

Live-Cell Time-Lapse Microscopy

Nikon TE300 Inverted Microscope (Live-Cell Time-Lapse System)
Location: Room B78 
Lenses
4X, 10X, 20X, 40X (all Long Working Distance)
2X (non-phase), 60X (non-phase, close working distance) and 100X oil (non-phase) available
Illumination options
Brightfield, Phase, Oblique, Fluorescence
Fluorescence Filter Cubes
DAPI, FITC, TRITC, CFP, YFP, (triple cube available for viewing only)
Fluorescence Filter Wheel:
DAPI, Pacific Blue, CFP, GFP, TRITC, CY5

Motorized XY Stage
Multiple inserts for well plates, 35mm dishes, standard glass slides. Positions can be saved for return sessions.

Environmental Chamber
Temperature and CO2 control surrounding entire microscope
Image Capture
-   Q-Imaging Retiga EX digital camera, high sensitivity monochrome camera 
1360 x 1036 pixel resolution
-   Single-shot color imaging through front camera port with Nikon D200 digital SLR camera

Workstation

Dell Core 2 Duo with ImagePro v.6.2, CD reader/writer, DVD reader/writer, USB and Firewire connections,  Dual 19” Flat Screens

Additional
Z-axis focusing controller with encoder, Automatic fluorescence shutter

Time-Lapse Capabilities
6D imaging (X, Y, Z, time, wavelength, positions)

Confocal Microscope

Leica TCS SPII Scanning Confocal Microscope
Location: Room G69A

Microscope:
            Leica DMRBE Inverted system
Lenses
10X dry, 25X oil, 40X oil, 100X oil
Illumination options
Brightfield, Fluorescence, DIC
Fluorescence Filter Cubes (for visible inspection):
            DAPI, FITC, TRITC, Dual FITC/TRITC
Fluorescence Excitation Wavelengths:
            458nm, 488nm, 568nm, 633nm
Image Capture
Leica PMT detection system – adjustable resolution, usually 1024 x 1024 pixels

Software

            ImagePro Plus 6.2
            Volocity 4.3
Helicon Focus
            Nikon ACT-1
Adobe Creative Suite 2
                        Photoshop
                        InDesign
                        GoLive
                        Illustrator
            Macromedia Flash
Macromedia Freehand
            Microsoft Office XP
PowerPoint
Excel
Word
Kodak PhotoDesk

2 Photon Microscope

Prairie Instruments Ultima 2 Photon Microscope:
Location: Room 281
Microscope:
            Fixed stage, upright system based on Olympus BX81
Lenses
10X, 20X, 40X and 60X dipping lenses
Laser
Coherent Chameleon XR Ti:Sapphire laser, 710-980nm wavelengths (variable)
Image Capture
4 detectors for Second Harmonic Generation and DAPI (457-487nm), GFP (503-537nm), YFP (525-570nm) and RFP (580-652nm)
Time Lapse Capabilities:
            5D imaging (X, Y, Z, time, wavelength)