Non-profit

Comm.

PROTEASE DIGEST ONLY

IN-GEL

Trypsin digest - large scale (96 well plate) 

$35

$70

Tryptic digest

$75

$150

AspN digest

$110

$220

IN-SOLUTION

Tryptic digest

$110

$220

LC-MS/MS, PROTEIN ID - Colloidal Blue Stain - submitted simultaneously

In-gel digest, nano LC-MS/MS on Orbitrap XL, db search & filtered Report

Small volume experiment (1 & 2)

each

$450

$900

Medium volume experiment (3 & 4)

each

$300

$600

Large volume experiment (5th & more)

each

$150

$300

Silver Stained LC-MS/MS, PROTEIN ID - Invitrogen SilverQuest only (cat #LC6070) submitted simultaneously

In-gel digest, nano LC-MS/MS on Orbitrap XL, db search & filtered Report

Small volume experiment (1 & 2)

each

$565

$1,125

Medium volume experiment (3 & 4)

each

$375

$750

Large volume experiment (5th & more)

each

$185

$370

Whole Proteome (Gel-LC-MS/MS) - per gel digest

$150

$300

Data set combine (per gel lane)

$130

$250

Custom Data Interpretation/Validation per hour (e.g., quantitative comparisons)

$65

$110

Characterization of PTMs - project dependent - consult with Manager

Phospho Enrichments (does not include LC/MS-MS)

$225

$450

Custom Database Creation - user provided sequences (per hour)

$70

$140

Database Creation - routine - NC

$0

$0

LC-MS/MS Data Analysis/Additional Computer Work - hourly rate

$70

$140

High Volume LC-MS/MS injection only (no digest or data analysis)

$75

$150

LC-MS/MS QC w/o Data Analysis per injection

$45

$90

LC-MS/MS QC setup (per group of samples submitted at the same time)

$90

$180

ESI of Intact Protein (where feasible)

Set up and Standards

$90

$180

Sample (one sample run and data collected)

$90

$180

Data Analysis/Additional Computer Work - hourly rate

$70

$140

RP-HPLC PEPTIDE MAP

Set up and Standards 

$120

$240

per sample injection

$120

$240

+cost/fraction (min 10 fractions) for MALDI MS

$30

$60

MATRIX ASSISTED LASER DESORPTION/IONIZATION (MALDI) MASS ANALYSIS

Routine Analysis

$50

$100

Special Handling

$90

$180

Additional analysis time per hour

$70

$140

SDS-PAGE Gels

1D gels - run and stain

$225

$450

Special Services

Ethanol Precip

$75

$150

SepPak c18 sample prep -up to 1 ml

$75

$150

Amicon Ultra Filtration

$150

$300

Bradford Assay

$150

$300

Methods Development, up to 2 hours’ time

$75

$150

Consultation with Manager/Director, per hour

$35

$35