User Fees

 

 

Non-profit

Comm.

 

PROTEASE DIGEST ONLY

 

 

IN-GEL

 

 

 

Trypsin digest - large scale (96 well plate) 

$35

$70

 

 

 

Tryptic digest

$75

$150

 

 

 

AspN digest

$110

$220

 

 

IN-SOLUTION

 

 

 

Tryptic digest

$110

$220

 

 

LC-MS/MS, PROTEIN ID - Colloidal Blue Stain - submitted simultaneously

 

 

In-gel digest, nano LC-MS/MS on Orbitrap XL, db search & filtered Report

 

 

Small volume experiment (1st & 2nd bands)

each

$450

$900

 

 

Medium volume experiment (3rd & 4th bands)

each

$300

$600

 

 

Large volume experiment (5th & more bands)

each

$150

$300

 

 

Silver Stained LC-MS/MS, PROTEIN ID - Invitrogen SilverQuest only (cat #LC6070) submitted simultaneously

 

 

In-gel digest, nano LC-MS/MS on Orbitrap XL, db search & filtered Report

 

 

Small volume experiment (1st & 2nd bands)

each

$565

$1,125

 

 

Medium volume experiment (3rd & 4th bands)

each

$375

$750

 

 

Large volume experiment (5th & more bands)

each

$185

$370

 

 

Whole Proteome (Gel-LC-MS/MS) - per gel digest

$150

$300

 

 

Data set combine (per gel lane)

$130

$250

 

 

Custom Data Interpretation/Validation per hour (e.g., quantitative comparisons)

$65

$110

 

 

Characterization of PTMs - project dependent - consult with Manager

 

 

Phospho Enrichments (does not include LC/MS-MS)

$225

$450

 

 

 

Custom Database Creation - user provided sequences (per hour)

$70

$140

 

 

 

Database Creation - routine - NC

$0

$0

 

 

 

LC-MS/MS Data Analysis/Additional Computer Work - hourly rate

$70

$140

 

 

High Volume LC-MS/MS injection only (no digest or data analysis)

$75

$150

 

 

LC-MS/MS QC w/o Data Analysis per injection

$45

$90

 

LC-MS/MS QC setup (per group of samples submitted at the same time)

$90

$180

 

 

ESI of Intact Protein (where feasible)

 

 

Set up and Standards

$90

$180

 

 

Sample (one sample run and data collected)

$90

$180

 

 

Data Analysis/Additional Computer Work - hourly rate

$70

$140

 

 

RP-HPLC PEPTIDE MAP

 

 

Set up and Standards 

$120

$240

 

 

per sample injection

$120

$240

 

 

+cost/fraction (min 10 fractions) for MALDI MS

$30

$60

 

 

MATRIX ASSISTED LASER DESORPTION/IONIZATION (MALDI) MASS ANALYSIS

 

 

Routine Analysis

$50

$100

 

 

Special Handling

$90

$180

 

 

Additional analysis time per hour

$70

$140

 

 

SDS-PAGE Gels

 

 

1D gels - run and stain

$225

$450

 

 

Special Services

 

 

Ethanol Precip

$75

$150

 

 

SepPak c18 sample prep -up to 1 ml

$75

$150

 

 

Amicon Ultra Filtration

$150

$300

 

 

Bradford Assay

$150

$300

 

 

Methods Development, up to 2 hours’ time

$75

$150

 

 

Consultation with Manager/Director, per hour

$35

$35