Vector Database

Vector Database

Bacterial Expression Plasmids

plasmid name	vendor	Tags	Ab
pRSETa	Invitrogen	N-6His	Amp	map	mcs	seq
pRSETb	Invitrogen	N-6His	Amp	map	mcs	seq
pRSETc	Invitrogen	N-6His	Amp	map	mcs	seq
pGEX-2T	GE Healthcare	N-GST	Amp	map	mcs	seq
pGEX-4T-1	GE Healthcare	N-GST	Amp	map	mcs	seq
pGEX-4T-2	GE Healthcare	N-GST	Amp	map	mcs	seq
pGEX-5X-1	GE Healthcare		Amp	map	mcs	seq
pGEX-5X-3	GE Healthcare	N-GST	Amp	map	mcs	seq
pGEX-6P1	GE Healthcare	N-GST	Amp	map	mcs	seq
pQE30	Qiagen	N-6His	Amp+Kan	map	mcs	seq
pQE31	Qiagen	N-6His	Amp+Kan	map	mcs	seq
pQE32	Qiagen	N-6His	Amp+Kan	map	mcs	seq
pQE50	Qiagen		Amp+Kan	map	mcs	seq
pET28a	Novagen		Kan	map	mcs	seq
pDUET	Novagen			map	mcs	seq
pMAL2CE	NEB	MBP	Amp	map	mcs	seq

Baculovirus Expression Vectors

Bac-to Bac method
plasmid name	vendor	tag	cleavage secretion
pFastBAC 1	Invitrogen				map	mcs	seq
pFastBAC Dual	Invitrogen				map	mcs	seq
pFastBAC HTA	Invitrogen	N-6His	Tev		map	mcs	seq
pFastBAC HTB	Invitrogen	N-6His	Tev		map	mcs	seq
pFastBAC HTC	Invitrogen	N-6His	Tev		map	mcs	seq
pFastBAC GST	Invitrogen	N-GST	Tev		map	mcs	seq
pFastBAC Flag	Invitrogen	N-FLAG	Tev		map	mcs	seq
pFastBAC Strep/His	Invitrogen	N-StrepII/6His	Tev		map	mcs	seq
pFastBAC FLAG/HTA	Invitrogen	N-FLAG/6His	Tev		map	mcs	seq
pFastBAC-HTB/FLAG	Invitrogen	N-6His/FLAG	Tev		map	mcs	seq
pFastBAC C-FLAG/His	Invitrogen	C-FLAG/His	Tev		map	mcs	seq

Baculogold method
plasmid name	vendor	tag	cleavage secretion
pVL 1392	Pharmingen				map	seq
pVL 1393	Pharmingen				map	seq
pAC HLT a	Pharmingen	N-6His	thrombin		map	seq
pAC HLT b	Pharmingen	N-6His	thrombin		map	seq
pAC HLT c	Pharmingen	N-6His	thrombin		map	seq
pAC GHLT a	Pharmingen	N-GST/6His	thrombin		map	seq
pAC GHLT b	Pharmingen	N-GST/6His	thrombin		map	seq
pAC GHLT c	Pharmingen	N-GST/6His	thrombin		map	seq
pAC GP67 a	Pharmingen			X	map	seq
pAC GP67 b	Pharmingen			X	map	seq
pAC GP67 c	Pharmingen			X	map	seq
pAc secG2T	Pharmingen	N-GST	thrombin	X	map	seq
pAc GP67b-His	Pharmingen	N-6His	Tev	X

pPolh FLAG	Sigma	FLAG			map	seq

Bac-N-Blue method
pBlueBAC 4.5 V5/HIS	Invitrogen	C-V5/6His			map	seq

Mammalian Expression Vectors

plasmid name	vendor	promoter	tag	selection
pcDNA3	Invitrogen	CMV		Neo	map	seq
pcDNA3-FLAG	Invitrogen	CMV	N-FLAG	Neo	map	seq
pcDNA3-HA	Invitrogen	CMV	N-HA	Neo	map	seq
pcDNA3-nGFP	Invitrogen	CMV	N-GFP	Neo	map	seq
pcDNA3-cGFP	Invitrogen	CMV	C-GFP	Neo	map	seq
pcDNA3-dsRED2	Invitrogen	CMV	N-dsRED2	Neo	map	seq
pcDNA3-nYFP	Invitrogen	CMV	N-YFP	Neo	map	seq
pcDNA3-nCFP	Invitrogen	CMV	N-CFP	Neo	map	seq
pcDNA3-GAL4(DBD)	Invitrogen	CMV	N-GAL4	Neo	map	seq
pcDNA3-ERHBDTM	Invitrogen	CMV	N-ERHBD	Neo	map	seq
pcDNA3.1-myc/his (A/B/C)	Invitrogen	CMV	C-myc/his	Neo	map	seq
pcDNA3.1-hygro	Invitrogen	CMV		hygro	map	seq

pcDNA4(TO)-myc/his (A/B/C)	Invitrogen	CMV-TRE	C-myc/his	Zeocin	map	seq
pcDNA4(TO)-FLAG	Invitrogen	CMV-TRE	N-FLAG	Zeocin	map	seq
pcDNA4(TO)-HA	Invitrogen	CMV-TRE	N-HA	Zeocin	map	seq
pcDNA4(TO)-EGFP	Invitrogen	CMV-TRE	N-EGFP	Zeocin	map	seq
pcDNA4(TO)-V5/FLAG	Invitrogen	CMV-TRE	C-V5/FLAG	Zeocin	map	seq
pcDNA4(TO)-cEGFP	Invitrogen	CMV-TRE			map	seq
pcDNA6/TR	Invitrogen			Blasticidin	map	seq

Lentivirus vectors

plasmid name	promoter	tag	selection
pLU-GFP	CMV		GFP	map	seq
pLU-DsRed2	CMV		DsRed2	map	seq
pLU-mCherry	CMV		mCherry	map	seq
pLU-YFP	CMV		YFP	map	seq
pLU-CFP	CMV		CFP	map	seq

pLU-TREmin-GFP	TREmin		GFP	map	seq

pLU-EF1-GFP	EF1		GFP	map	seq
pLU-EF1-mCherry	EF1		mCherry	map	seq
pLU-EF1-FFluc	EF1			map	seq
pLU-EF1-Ffluc/mCherry	EF1		mCherry	map	seq

pLU-UbiC-GFP	UbiC		GFP	map	seq
pLU-UbiC-mCherry	UbiC		mCherry	map	seq

pLU-T-CMV-pPuro	TRE-CMV	N-FLAG	Puro	map	seq
pLU-T-CMV-pBlast	TRE-CMV	N-FLAG	Blasticidin	map	seq
pLU-T-CMV-pGFP	TRE-CMV	N-FLAG	GFP	map	seq

pLU-TREmin-pPuro	TREmin	N-FLAG	Puro	map	seq

pLU-T-EF1-pPuro	TRE-EF1	N-FLAG	Puro	map	seq
pLU-T-EF1-pBLAST	TRE-EF2	N-FLAG	Blasticidin	map	seq
pLU-T-EF1-pGFP	TRE-EF3	N-FLAG	GFP	map	seq

pLU-tTR-KRAB-iRed2	CMV		DsRED2	map	seq
pLU-tTR-KRAB-iBlast	CMV		Blasticidin	map	seq
pLU-tTR-KRAB-iCherry	CMV		mCherry	map	seq
pLU-rtTA3-iCherry	CMV		mCherry	map	seq

pLKO.1	U6		puro	map	seq
pLKO-tGFP	CMV		turboGFP	map	seq
pGIPZ	CMV		Puro	map	seq

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Featured Image: Horner Brass Microscope

The microscope in the image belonged to William E. Horner, M.D., a collaborator with Caspar Wistar, M.D., in the early 1800s.

Dr. Horner, a lecturer at the University of Pennsylvania, was a pioneer of the use of microscopes in anatomical and medical research. He authored Special Anatomy and Histology, a seminal text on the subject.

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