Protein Expression and Libraries Facility

The Wistar Institute
Room G68
3601 Spruce Street
Philadelphia, PA 19104
Tel. 215-898-3814
Fax 215-898-3868

Facility Hours:  8:00 a.m. - 6:00 p.m., Monday through Friday

Paul Lieberman, Ph.D.
Scientific Director

David C. Schultz Ph.D.
Facility Director
215-495-6885

Henry Hoff
Wistar Research Assistant

Barbara Niedziolka
Research Assistant I

Aastha Puri
Research Assistant I

Grace Heck
Research Assistant I

Introduction

The Protein Expression and Libraries Facility is a center of technological excellence dedicated to viral vector production and the expression and purification of recombinant proteins.  The facility staff provide consultation, training and technical support to the scientific community in the use of prokaryotic and eukaryotic expression vector systems for recombinant protein production.  The major services offered include:

1. Recombinant DNA engineering,
2. Viral vector production (i.e. baculovirus and retrovirus),
3. Analytical and preparative scale expression of nascent or epitope-tagged recombinant proteins,
4. Protein purification.

These goals are accomplished by a centralized laboratory, which allows for dedicated high-throughput, economy of scale, virus production and protein expression services, including quality assurance and control procedures to ensure efficient, consistent production and purification of recombinant proteins and viral vectors.  Many recombinant proteins produced by the facility have been used for crystallization efforts, studies designed to investigate enzymatic properties, structure-function relationships between protein-protein, protein-nucleic-acid, and protein-small molecule interactions, custom antibody production, experimental cancer vaccines, and development of miniaturized assays for small molecule screening.