| Probe |
Ex (nm) |
Em (nm) |
MW |
Notes |
| Reactive
and conjugated probes |
| Hydroxycoumarin |
325 |
386 |
331 |
Succinimidyl
ester |
| Aminocoumarin |
350 |
445 |
330 |
Succinimidyl
ester |
| Methoxycoumarin |
360 |
410 |
317 |
Succinimidyl
ester |
| Cascade
Blue |
375;400 |
423 |
596 |
Hydrazide |
| Lucifer
yellow |
425 |
528 |
|
|
| NBD |
466 |
539 |
294 |
NBD-X |
| R-Phycoerythrin
(PE) |
480;565 |
578 |
240,000 |
|
| PE-Cy5
conjugates |
480;565;650 |
670 |
|
aka Cychrome,
R670, Tri-Color, Quantum Red |
| PE-Cy7
conjugates |
480;565;743 |
767 |
|
|
| Red 613 |
480;565 |
613 |
|
PE-Texas
Red |
| Fluorescein |
495 |
519 |
389 |
FITC;
pH sensitive |
| BODIPY-FL |
503 |
512 |
|
|
| Cy3 |
512;552 |
565,615 |
|
|
| TRITC |
547 |
572 |
444 |
TRITC |
| X-Rhodamine |
570 |
576 |
548 |
XRITC |
| Lissamine
Rhodamine B |
570 |
590 |
|
|
| PerCP |
490 |
675 |
|
Peridinin
chlorphyll protein |
| Texas
Red |
589 |
615 |
625 |
Sulfonyl
chloride |
| Cy5 |
625-650 |
670 |
|
|
| Cy7 |
743 |
767 |
1,001 |
|
| Allophycocyanin
(APC) |
650 |
660 |
104,000 |
|
| TruRed |
490,675 |
695 |
|
PerCP-Cy5.5
conjugate |
| APC-Cy7
conjugates |
650;755 |
767 |
|
PharRed |
| Nucleic
acid probes |
| Hoechst
33342 |
343 |
483 |
616
|
AT-selective
|
| DAPI |
345 |
455 |
|
AT-selective
|
| Hoechst
33258 |
345 |
478 |
624 |
AT-selective
|
| SYTOX
Blue |
431 |
480 |
~400 |
DNA
|
| Chromomycin
A3 |
445 |
575 |
|
CG-selective
|
| Mithramycin |
445 |
575 |
|
|
| YOYO-1 |
491 |
509 |
1270.65 |
|
| SYTOX
Green |
504 |
523 |
~600
|
DNA
|
| SYTOX
Orange |
547 |
570 |
~500
|
DNA
|
| Ethidium
Bromide |
493 |
620 |
394.31
|
|
| 7-AAD |
546 |
647 |
|
7-aminoactinomycin
D, CG-selective |
| Acridine
Orange |
503 |
530/640 |
|
DNA/RNA |
| TOTO-1,
TO-PRO-1 |
509 |
533 |
|
Vital
stain, TOTO: Cyanine Dimer
TO-PRO: Cyanine Monomer |
| Thiazole
Orange |
510 |
530 |
|
|
| Propidium
Iodide (PI) |
536 |
617 |
668.4 |
|
| TOTO-3,
TO-PRO-3 |
642 |
661 |
|
|
| LDS 751 |
543;590 |
712;607 |
471.98
|
DNA
(543ex/712em), RNA (590ex/607em) |
| Cell
function probes |
| Indo-1 |
361/330 |
490/405 |
1010 |
AM ester.
Low/High Ca++, |
| Fluo-3 |
506 |
526 |
855 |
AM ester.
pH > 6 |
| DCFH |
505 |
535 |
529 |
2'7'Dichorodihydrofluorescein,
oxidized form |
| DHR |
505 |
534 |
346 |
Dihydrorhodamine
123, oxidized form,
light catalyzes oxidation |
| SNARF |
548/579 |
587/635 |
|
pH 6/9 |
| Fluorescent
Proteins |
| Y66F |
360 |
508 |
|
|
| Y66H |
360 |
442 |
|
|
| EBFP |
380 |
440 |
|
(Clonetech)
Quantum yield 0.18 |
| Wild Type |
396,475 |
508,503 |
|
|
| GFPuv |
385 |
508 |
|
(Clonetech) |
| ECFP |
434 |
477 |
|
(Clonetech)
Quantum yield 0.40 |
| Y66W |
436 |
485 |
|
|
| S65A |
471 |
504 |
|
|
| S65C |
479 |
507 |
|
|
| S65L |
484 |
510 |
|
|
| S65T |
488 |
511 |
|
|
| EGFP |
489 |
508 |
|
(Clonetech)
Quantum yield 0.60 |
| EYFP |
514 |
527 |
|
(Clonetech)
Quantum yield 0.61 |
| DsRed |
558 |
583 |
|
(Clonetech)
Quantum yield 0.29 |
| Other
probes |
| Monochlorobimane |
380 |
461 |
226 |
Glutathione
probe |
| Calcein |
496 |
517 |
623 |
pH >
5 |