|
PARAMETER |
MEASUREMENT
METHOD OR PROBE IF USED |
|
|
|
|
Intrinsic
Parameters |
(no
probes needed) |
|
Cell
size |
Forward
light scatter |
|
Cytoplasmic
granularity |
Right
angle (or side) light scatter |
|
Cell
shape and aggregation |
Pulse
shape analysis |
|
Autofluorescence |
Fluorescence |
|
|
|
Extrinsic Parameters |
|
Antigens
|
Fluorescence
(labeled antibodies) |
DNA
content
|
Fluorescence
(e.g., PI) |
Nucleic
acid sequence
|
Fluorescence
(e.g., EGFP, β-gal) |
RNA
content
|
Fluorescence
(e.g., AO) |
Surface
sugars
|
Fluorescence
(labeled lectins) |
|
|
|
Extrinsic Functional Parameters |
|
Membrane
integrity (viability)
|
Fluorescence
(PI) |
DNA
degradation (apoptosis)
|
Fluorescence
(e.g., PI) |
DNA
synthesis
|
Fluorescence
(anti-BrUdR antibodies) |
Cytokines
|
Fluorescence
(labeled antibodies) |
Cell
division
|
Fluorescence
(CFSE) |
Conjugate
formation
|
Fluorescence
(HE, calcein, etc.) |
Surface
receptors
|
Fluorescence
(labeled ligands) |
Membrane
organization
|
Fluorescence
(Annexin) |
Intracellular
receptors
|
Fluorescence
(labeled ligands) |
Cytoplasmic
Ca++
|
Fluorescence
ratio (indo-1); fluorescence (fluo-3) |
Intracellular
pH
|
Fluorescence
ratio (e.g., SNARF) |