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Genomics/Microarray Facility
Services (cDNA Array Production)
Microarray
Facility
Contact: Celia Chang, Ph.D.
Tel (215) 898-3902
Fax (215) 898-4521
The Microarray facility was established in March
of 1999. Dr. Celia Chang, a Ph.D. biochemist, has been working for
the facility since 1999 and has the primary responsibility for array
production and facility management. Dr. Chang is assisted by Irina
Feldman, research technician and Wen-Hwai Horng whose primary responsibility
is in basic array analysis and all aspects of clone tracking.
Forty thousand sequence verified human clones and
12,000 mouse clones have been purchased by the facility from Research
Genetics and 15,000 full length mouse clones derived from mouse
early embryos have been acquired from the NIA. PCR products for
the complete yeast genome have been acquired as a joint effort with
Fox ChaseCancerCenter and Wistar yeast researchers. Click
here to see a list of production arrays for sale.
Quality Control
DNA fragments used to generate the cDNA
arrays are produced using PCR and universal primers to amplify cloned
insert from bacterial cultures or from purified plasmid DNA. PCR
products are visualized on agarose gels, scanned using a fluoroimager
and the concentration of each PCR product is determined using
ImageQuant. A record of the scanned images of each batch of arrays
is archived. Plates that fall below the DNA concentration required
for spotting are re-amplified, and clones with more than one band
are identified. Arrays produced from one batch of PCR products are
numbered sequentially until that batch of DNA is completed. Click here to view diagram of the steps involved in making
arrays.
Samples from each printing run are tested
for spotting consistency by hybridization with a 33P
labeled cDNA probe. Every effort is made to provide sequentially
printed arrays when multiple arrays are purchased. The number of
total arrays you will require should be taken into consideration
when deciding how many arrays to order.
Custom
Arrays
Custom arrays of clones provided by the
user or a selection of facility clones can also be constructed.
Inquire for charges.
Hybridization Test Filters
Test filters will be provided for new users
when available in order to test your hybridization and washing procedures
Other Services:
- Protocols:
RNA probe preparation, hybridization,
RNA amplification.
- Trouble shooting:
Send an email with your questions arraysupport@wistar.org
- Training:
By appointment only, hourly cost basis.
- Grant applications:
Core descriptions and facility generated
array figures can be provided for grant applications with sufficient
notice. Email lshowe@wistar.org
with questions.
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