Labor charges are $50 per hour for Wistar Investigators, $60 per hour for non-Wistar academic users and $70 per hour for commercial users.
Description |
Type |
Wistar Investigator |
Illumina whole -genome expression for Human and mouse
total RNA to be amplified, hybridized, washed & scanned |
6 Sample
Beadchip
|
$1,920/Chip
($320/sample) |
8 Sample Beadchip
(RefSeq only) and
Ratref_seq_12
Beadchip |
$2,080/Chip
($260/sample) |
| Human HT-12 beadchip |
$2,820/chip
($235/sample) |
SNP Genotyping Analysis |
mouse low density (Ld) linkage panel,
96 samples |
$4,512/Array
($47/sample) |
Illumina MicroRNA expression profiling,Human /Mouse samples. |
Beadchip arrays,
24 samples** |
$220/sample |
Illumina DASL Gene Expression for degraded RNA samples with RNA isolation |
whole_Genome DASL Assay,
24 samples* * |
$275/sample |
Illumina DASL Gene Expression for degraded RNA samples without RNA isolation |
Whole_Genome DASL Assay,
24 samples** |
$225/sample |
Illumina Infinium Whole-genome SNP genotyping and Copy Number Variation(CNV) Analysis |
Upon request |
Upon request |
| Illumina Infinium Methylation profiling with Bisulfite conversion |
HumanMethylation27 Beadchips,
24 samples** |
$355/sample |
| Illumina Infinium Methylation profiling without Bisulfite conversion |
HumanMethylation27 Beadchips,
24 samples** |
$330/sample |
| RNA isolation |
Tri-reagent or RNAqueous-Micro kit |
$30/sample |
RNA isolation |
Paxgene kit |
$36/sample |
RNA isolation |
High Pure RNA Paraffin kit |
$50/sample |
RNA Amplification |
Ambion or Epicentre |
$75/sample |
| ABI 7900 HT Fast RealTime PCR System |
miRNA profiling using the ABI TagMan low Density arrays |
$600/sample |
| RealTime PCR |
Upon request |
| ABI 3130xl Sequencing system |
DNA samples |
$7 per sequence |
| Sequencing Rxn done by user |
Wistar Investigator only, $5 per lane |
| Microsatellite analysis , 5 loci |
$20/sample |
*Genomic scale expression profiling requires time and an understanding of the inherent limitations of microarrays we recommend that each investigator take time before submitting samples to discuss the project with one of our array specialists in the lab. This will help predict the number of replicates needed and estimate the time and cost of a project and improve the chances that the arrays used will yield results you can have confidence in.
**Assays must include 24 samples. For less than 24 samples, please contact Celia Chang at chang@wistar.org.
| Illumina Genome Analyzer |
| Illumina Genome Analyzer- Sample Prep + Sequencing |
Wistar Investigator* |
ChIP-seq sample prep + 36bp Sequencing
(+ $900 for 72bp) |
$1,425 |
Small RNA sample prep + 36bp Sequencing
(+ $900 for 72bp) |
$1,475 |
Genomic DNA sample prep + 36bp Sequencing
(+ $900 for 72bp) |
$1,425 |
mRNA-seq sample prep + 36bp Sequencing
(+ $900 for 72bp) |
$1,425 |
Digital Gene Expression: Tag Profiling sample prep + 36bp Sequencing (+ $900 for 72bp) |
$1,325 |
Paired-end Sample Prep + 36bp 2 Read Sequencing |
$2,475 |
Paired-end Sample Prep + 72bp 2 Read Sequencing |
$4,350 |
Illumina Genome Analyzer- Sequencing Only |
Wistar Investigator* |
36bp Sequencing only (Client prepares Samples) |
$950 |
| 72bp Sequencing only (Client prepares Samples) |
$1,900 |
Paired-end 36bp 2 Read Sequencing Only
(Client prepares Samples) |
$1,975 |
Paired-end 72bp 2 Read Sequencing Only
(Client prepares Samples) |
$3,825 |
| Illumina Genome Analyzer-Mutliplex Sequencing |
Wistar Investigator* |
| Multiplex Sample Prep Only -per sample |
$575 |
Multiplex 36bp Sequencing Only- per lane
(up to 4 samples) |
$1,175 |
Multiplex 72bp Sequencing Only- per lane
(up to 4 samples) |
$2,100 |
