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Genomics/Microarray Facility

User Fees

Please e-mail Celia Chang if you have specific questions.

Labor charges are $50 per hour for Wistar Investigators, $60 per hour for non-Wistar academic users and $77.50 per hour for commercial users.

There is a 20% surcharge for Non-Wistar Academic users and a 55% surcharge for commercial users on all facility fees.

Please note that the fees for bioinformatics is seprate and can be viewed by clicking here.

Description
Type
Wistar Investigator
Illumina whole -genome expression for Human and mouse
total RNA to be amplified (Ambion or Epicentre kits), hybridized, washed & scanned
6 Sample
Beadchip
$1,920/Chip
($320/sample)
8 Sample Beadchip
(RefSeq only) and
Ratref_seq_12
Beadchip

$2,080/Chip
($260/sample)
for 8 samples

$3,120
for 12 samples

Human HT-12 beadchip $2,820/chip
($235/sample)
Illumina whole -genome expression for Human and mouse
total RNA to be amplified and labled (NuGEN Kits) hybridized, washed & scanned
6 Sample
Beadchip
$2,130/Chip
($355/sample)
8 Sample Beadchip
(RefSeq only) and
Ratref_seq_12
Beadchip

$2,360/Chip
($295/sample) for 8 samples ,

$3,540 for 12 samples
Human HT-12 beadchip $3,240/chip
($270/sample)

SNP Genotyping Analysis

mouse low density (Ld) linkage panel,
96 samples

$4,512/Array
($47/sample)

Illumina MicroRNA expression profiling,Human /Mouse samples.

Beadchip arrays,
24 samples**

$220/sample

Illumina DASL Gene Expression for degraded RNA samples with RNA isolation 

whole_Genome DASL Assay,
24 samples* *

$275/sample  

Illumina DASL Gene Expression for degraded RNA samples without RNA isolation

Whole_Genome DASL Assay,
24 samples**

$225/sample 

Illumina  Infinium Whole-genome SNP genotyping and Copy Number Variation(CNV) Analysis

Upon request 

Upon request 

Illumina Infinium Methylation profiling with Bisulfite conversion HumanMethylation27 Beadchips,
24 samples** 
$355/sample 
Illumina Infinium Methylation profiling without Bisulfite conversion HumanMethylation27 Beadchips,
24 samples**
$330/sample
RNA isolation Tri-reagent or RNAqueous-Micro kit $30/sample

RNA isolation

Paxgene kit

$36/sample

RNA isolation

High Pure  RNA Paraffin kit

$50/sample

RNA Amplification

Ambion or Epicentre

$75/sample

RNA Amplification plus labeling NuGEN $110/sample
ABI 7900 HT Fast RealTime PCR System miRNA profiling using the ABI TagMan low Density arrays $600/sample
RealTime PCR Upon request 
ABI 3130xl Sequencing system DNA samples $7 per sequence
Sequencing Rxn done by user Wistar Investigator only, $5 per lane
Microsatellite analysis , 5 loci $20/sample

*Genomic scale expression profiling requires time and an understanding of the inherent limitations of microarrays we recommend that each investigator take time before submitting samples to discuss the project with one of our array specialists in the lab. This will help predict the number of replicates needed and estimate the time and cost of a project and improve the chances that the arrays used will yield results you can have confidence in.

**Assays must include 24 samples. For less than 24 samples, please contact  Celia Chang at chang@wistar.org.


For Information about Solexa Sequencing, please email the Solexa specialist at solexa@wistar.org.

Please note that the fees for bioinformatics is seprate and can be viewed by clicking here.

Illumina Genome Analyzer
Illumina Genome Analyzer- Sample Prep + Sequencing Wistar Investigator*

ChIP-seq sample prep + 36bp Sequencing
(+ $300 for 72bp)

$1,425

Small RNA sample prep + 36bp Sequencing
(+ $300 for 72bp)

$1,475

Genomic DNA sample prep + 36bp Sequencing
(+ $300 for 72bp)

$1,425

mRNA-seq sample prep + 36bp Sequencing
(+ $300 for 72bp)

$1,425

Digital Gene Expression: Tag Profiling sample prep + 36bp Sequencing (+ $300 for 72bp)

$1,325

Paired-end Sample Prep + 36bp 2 Read Sequencing

$2,100

Paired-end Sample Prep + 72bp 2 Read Sequencing

$2,500

Illumina Genome Analyzer- Sequencing Only

Wistar Investigator*

36bp Sequencing only (Client prepares Samples)

$950

72bp Sequencing only (Client prepares Samples) $1,250
Paired-end 36bp 2 Read Sequencing Only
(Client prepares Samples)
$1,550
Paired-end 72bp 2 Read Sequencing Only
(Client prepares Samples)
$1,950
Illumina Genome Analyzer-Mutliplex Sequencing Wistar Investigator*
Multiplex Sample Prep Only -per sample $650
Multiplex 36bp Sequencing Only- per lane
(up to 4 samples)
$1,175
Multiplex 72bp Sequencing Only- per lane
(up to 4 samples)
$1,475

* There is a 20% surcharge for Non-Wistar Academic users and a 55% surcharge for commercial users on all facility fees.

Inquire about uneven Paired-end Sequencing reads (36bp one direction, 72bp other direction), or Multiplexing Paired-end Sequencing.

 

 
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