Hybridization / Imaging Services

Gene expression analysis using microarrays including every step from mRNA amplification (if necessary) and labeling through hybridization, washing, and image acquisition is available at the Wistar Core lab. Please take a moment to check out our flow chart of hybridization and washing steps involved in filter microarray projects.
The investigator is asked to choose the facility array that best suits their needs and provide information about the quantity and quality of the RNA being submitted. The average time required for hybridizations, starting with sample submission and ending with an image of the array is 10 working days, but this time will vary based on the quality and quantity of RNA.

This form should be printed out and accompany all RNA samples submitted for analysis

Samples submitted for analysis should be provided in eppendorf tubes clearly labeled with the following information:

1) Species of RNA sample.
2) Whether or not the RNA has been DNAse treated.
3) Concentration of RNA.
4) Whether the sample is total or polyA RNA.

**The sample RNA form, and a picture of the gel on which the RNA was run should accompany the RNA.

After scanning the array we can analyze the image or we can return it and allow you to do the analysis on our Dell Pentium 4 using Arrayvision or Imagene (with subscription purchase), or with your own hardware and software.

Please contact Celia Chang in order to arrange hybridization of your mRNA samples. Our lab will radioactively label your RNA, hybridize and wash the arrays according to our lab protocols, and then expose the array on a phosphorimager screen and scan the image at 50 micron/pixel resolution.