Genomics/Microarray Facility

Libraries

The Wistar Genomics Facility has four unique cDNA clone libraries stored as glycerol stocks in 96 well plates at -80 in our facility freezers. Two of the libraries are human clones and two of the libraries are mouse clones. The libraries are characterized as follows. Please fill this form and return with your request.

1. GF200.
The 40k set" This is the original sequence verified I.M.A.G.E. consortium set of human clones that was used to create all of the Genefilter arrays from Research Genetics. The Wistar Institute purchased plates 1-417, and will make these 40,000 clones available to researchers. The set contains 3900 unknown genes (not associated with a cluster ID) and there are multiple accession numbers for some of the cluster ID's. The average insert size is 1.24 kb.*These clones were used exclusively for Wistar Arrays HA01 and HA02

2. HsKGV.
The Human Known Gene Set" This clone library was rearrayed by Research Genetics using the sequence verified GF 200 clones so that only clones that had been affirmatively associated with a human cluster ID in unigene would be included, and only one accession number per Cluster ID would be included. The set contains 78 plates and just shy of 7500 clones with an average insert size of 1.45 kb *These clones make up a large percentage of the array elements on the Wistar human arrays HA03, HA04, and HA05.

3. NIA Developmental Mouse Clone set
NIA 15k set" The National institute of Aging prepared this clone library was and made it available free of cost to researchers willing to provide access to the clones to others at their institution through arrays and library storage. The link to the NIA site is included here. The average insert size is 1.5 kb and up to 50% of the NIA clones are derived from novel genes. The clone set includes 15,000 "unique" cDNA clones which have been rearrayed from 52,374 ESTs from pre- and periimplantation embryos, E12.5 female gonad/mesonephros, and newborn ovary. Unfortunately the annotation for theses genes has lagged behind the human clone sets, but the complete Fasta Formatted sequence files are available for all clones and their information will be updated as it becomes available. These clones were used exclusively for the Wistar array MA07 (9600 array elements), and will be used in the upcoming MA09 and MA10 arrays.

4. Research Genetics Mouse Clones. Work in Progress. Clones available by special request. If you find a clone that you are interested in obtaining from the Genomics Core Facility please print out the sheet and fill out the information about the clone including the following information or include this information as an attached spreadsheet..

1) Clone Set
2) Plate number
3) Row Letter (A->H)
4) Column Number (1->12)
5) Accession number
6) Unigene (If applicable)

The easiest way to do this is to copy the rows from the excel spreadsheets that you need and compile them into a list of clones that you're interested in. Then we will find the plate remove a stab and grow it overnight in Terrific broth with 8% glycerol.

The charges for this will be minimal and based on the time required to locate, thaw, re-freeze and grow each clone.

We suggest that all clones be sequence verified before using the clone for further research. Although all of these libraries were provided to us and certified as the correct sequences we have found many inconsistencies with the sequences as have other core facilities and researchers so it is always safer to sequence verify first.