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Microscopy Facility
Widefield Microscopes
 Nikon E600 Upright Microscope
Location: Room B78
Lenses:
2X, 4X, 10X, 20X, 40X, 60X and 100X oil
Illumination options:
Brightfield, Darkfield, Fluorescence, Polarization
Fluorescence Filter Cubes:
DAPI, FITC, TRITC, CFP, YFP, (triple cube available for viewing only)
Image Capture:
Spot RT Slider digital camera w/ ImagePro Plus software for fluorescence monochrome capture - 1600 x 1200 pixel resolution.
Nikon DXM 1200F digital camera w/ Nikon ACT1 software for color capture –
Variable pixel resolution, 1280 x 1024 pixels standard
Workstation
Pentium 4 Dell PC with ImagePro v.4.5, 48X CD reader/writer, Zip 250 drive, front USB port
Leica DMRE Upright Microscope (For Fluorescence)
Location: Room B80
Lenses:
2.5X, 10X, 20X, 40X and 100X oil
Illumination options:
Brightfield, Fluorescence,
Fluorescence Filter Cubes:
DAPI, GFP/Aqua, HQ FITC, Piston GFP, HQ R, CY5.5, G/R (for viewing only), GFP/DsRed FRET
Image Capture:
Hammamatsu ORCA digital camera w/ ImagePro Plus software for fluorescence monochrome capture – 1344 x 1024 or 672 x 512 pixel resolution.
Workstation
Pentium 4 Dell PC with ImagePro v.6.2, 48X CD reader/writer, Zip 250 drive, front USB port
Zeiss Axioplan Upright Microscope (for Brightfield)
Location: Room B80
Lenses: 10X, 20X, 40X and 100X oil
Illumination options: Brightfield,
Image Capture:
Nikon DXM 1200F digital camera w/ Nikon ACT1 software for color capture –
Variable pixel resolution, 1280 x 1024 pixels standard
Workstation
Pentium 4 Dell PC with 48X CD reader/writer, Zip 250 drive, front USB port (share with SMZ800 steromicroscope)
Nikon TE300 Inverted Microscope (Live-Cell Time-Lapse System)
Location: Room B78
Lenses:
4X, 10X, 20X, 40X (all Long Working Distance)
2X (non-phase), 60X (non-phase, close working distance) and 100X oil (non-phase) available
Illumination options:
Brightfield, Phase, Oblique, Fluorescence
Fluorescence Filter Cubes:
DAPI, FITC, TRITC, CFP, YFP, (triple cube available for viewing only)
Fluorescence Filter Wheel:
DAPI, Pacific Blue, CFP, GFP, TRITC, CY5
Motorized XY Stage
Multiple inserts for well plates, 35mm dishes, standard glass slides. Positions can be saved for return sessions.
Environmental Chamber
Temperature and CO2 control surrounding entire microscope
Image Capture:
- Q-Imaging Retiga EX digital camera, high sensitivity monochrome camera 1360 x 1036 pixel resolution
- Single-shot color imaging through front camera port with Nikon D200 digital SLR camera
Workstation
Dell Core 2 Duo with ImagePro v.6.2, CD reader/writer, DVD reader/writer, USB and Firewire connections, Dual 19” Flat Screens
Additional
Z-axis focusing controller with encoder, Automatic fluorescence shutter
Time-Lapse Capabilities
6D imaging (X, Y, Z, time, wavelength, positions)
Nikon TE2000 Inverted Microscope
Location: Room B78
Lenses:
4X, 10X, 20X, 40X (all Long Working Distance)
2X (non-phase), 60X (non-phase, close working distance) and 100X oil (non-phase) available
Illumination options:
Brightfield, Phase, Oblique, Fluorescence
Fluorescence Filter Cubes:
DAPI, CFP, FITC, TRITC, Texas Red
Image Capture:
- Q-Imaging Retiga EX digital camera, high sensitivity monochrome camera
1360 x 1036 pixel resolution
- Nikon DXM 1200F digital camera w/ Nikon ACT1 software for color capture –
Variable pixel resolution, 1280 x 1024 pixels standard
- Single-shot color imaging available through front camera port with Nikon D200 digital SLR camera
Workstation
Pentium 4 Dell PC with ImagePro v.6.2, CD reader/writer, DVD reader/writer, USB and Firewire connections,
 Nikon SMZ800 Stereo Microscope
Location: Room B80
Magnification Range: 1X objective with 1X – 6.3X variable zoom
Illumination options: Brightfield, Darkfield, Polarization, Oblique Illumination, Episcopic Lighting, Fiber optic light tubes
Fluorescence: GFP possible with Illumatool 9900 system
Image Capture: - Nikon DXM 1200F digital camera w/ Nikon ACT1 software for color capture –
Variable pixel resolution, 1280 x 1024 pixels standard
Workstation
Pentium 4 Dell PC with 48X CD reader/writer, Zip 250 drive, front USB port (share with Zeiss Axioplan back-up upright microscope)
Live-Cell Time-Lapse Microscopy
Nikon TE300 Inverted Microscope (Live-Cell Time-Lapse System)
Location: Room B78
Lenses:
4X, 10X, 20X, 40X (all Long Working Distance)
2X (non-phase), 60X (non-phase, close working distance) and 100X oil (non-phase) available
Illumination options:
Brightfield, Phase, Oblique, Fluorescence
Fluorescence Filter Cubes:
DAPI, FITC, TRITC, CFP, YFP, (triple cube available for viewing only)
Fluorescence Filter Wheel:
DAPI, Pacific Blue, CFP, GFP, TRITC, CY5
Motorized XY Stage
Multiple inserts for well plates, 35mm dishes, standard glass slides. Positions can be saved for return sessions.
Environmental Chamber
Temperature and CO2 control surrounding entire microscope
Image Capture:
- Q-Imaging Retiga EX digital camera, high sensitivity monochrome camera
1360 x 1036 pixel resolution
- Single-shot color imaging through front camera port with Nikon D200 digital SLR camera
Workstation
Dell Core 2 Duo with ImagePro v.6.2, CD reader/writer, DVD reader/writer, USB and Firewire connections, Dual 19” Flat Screens
Additional
Z-axis focusing controller with encoder, Automatic fluorescence shutter
Time-Lapse Capabilities
6D imaging (X, Y, Z, time, wavelength, positions)
Confocal Microscope
Leica TCS SPII Scanning Confocal Microscope
Location: Room G69A
Microscope:
Leica DMRBE Inverted system
Lenses:
10X dry, 25X oil, 40X oil, 100X oil
Illumination options:
Brightfield, Fluorescence, DIC
Fluorescence Filter Cubes (for visible inspection):
DAPI, FITC, TRITC, Dual FITC/TRITC
Fluorescence Excitation Wavelengths:
458nm, 488nm, 568nm, 633nm
Image Capture:
Leica PMT detection system – adjustable resolution, usually 1024 x 1024 pixels
Software
ImagePro Plus 6.2
Volocity 4.3
Helicon Focus
Nikon ACT-1
Adobe Creative Suite 2
Photoshop
InDesign
GoLive
Illustrator
Macromedia Flash
Macromedia Freehand
Microsoft Office XP
PowerPoint
Excel
Word
Kodak PhotoDesk
2 Photon Microscope
Prairie Instruments Ultima 2 Photon Microscope:
Location: Room 281
Microscope:
Fixed stage, upright system based on Olympus BX81
Lenses:
10X, 20X, 40X and 60X dipping lenses
Laser:
Coherent Chameleon XR Ti:Sapphire laser, 710-980nm wavelengths (variable)
Image Capture:
4 detectors for Second Harmonic Generation and DAPI (457-487nm), GFP (503-537nm), YFP (525-570nm) and RFP (580-652nm)
Time Lapse Capabilities:
5D imaging (X, Y, Z, time, wavelength)
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