Monoclonal Antibody to Human Daxx

Inventors:    Gerd G. Maul and Alexey Sotnikov

Tech ID:    MG-01RR

Description: Wistar researchers have developed a monoclonal antibody (isotype IgG1) named hDaxxab, which binds to human Daxx. Recently, Daxx protein has been found to be involved in apoptosis and to interact with a number of proteins (e.g., Fas, CENT-P, DNA-methylase, Pax3, PML) that are involved in regulation of cell growth. In immunohistochemical staining, the antibody specifically localizes to nuclear domain 10 (ND10) of primate cells. Using human Daxx fragments of various sizes, the epitope recognized by the antibody was found between aa 515 and 535. Western blot analysis demonstrates a single band in total cell extracts. In comparison to other available polyclonal antibodies to Daxx, this monoclonal antibody does not label cytoplasm. Additionally, no centromeric binding is observed, except during G2-phase of the cell cycle when Daxx is found beside centromeric domains. All the Daxx-specific nuclear sites are also labeled by anti-PML and represent ND10 rather than centromeres.

Keywords: Research tool, research reagent, monoclonal antibody, cancer.

Applications: The monoclonal antibody hDaxxab works in immunoblotting (i.e., Western blot), immunohistochemistry in primate cells, and immunoprecipitation.

References: Ishov, A.M., Sotnikov, A.G., Negorev, D., Vladimirova, O.V., Neff, N., Kamitani, T., Yeh, E.T., Strauss, J.F., 3rd, and Maul, G.G. PML is Critical for ND10 Formation and Recruits the PML-interacting Protein Daxx to this Nuclear Structure When Modified by SUMO-1. J. CELL BIOL. 147:221-234 (Oct. 18, 1999).

Licensing Opportunity: This technology is available for licensing on a non-exclusive basis for the development and sale of research reagents.

Contact:

Meryle J. Melnicoff, Ph.D.
Director, Business Development The Wistar Institute
Phone: (215) 898-0049
melnicoff@wistar.org