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Monoclonal Antibodies That
Bind to Cell Surface Antigens on Human Embryonal Carcinoma Cells
Inventors:
Barbara Knowles, Davor Solter, Peter Andrews
Tech ID:
WST-20RR
Introduction:
Wistar researchers have developed monoclonal antibodies against
five cell surface markers that are useful in the identification
and characterization of human embryonic stem cells and monitoring
their differentiation. Investigation of the function and expression
of developmentally regulated cell surface antigens may yield clues
to the control of cell differentiation, not only during embryogenesis
but also during oncogenesis. These monoclonal antibodies bind to
cell surface antigens SSEA-1 (stage-specific mouse embryonic antigen),
SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81.
Origin:
MC-480 (SSEA-1) hybridomas were produced by fusing P3-X63-Ag8
mouse myeloma cells with splenocytes from a BALB/c mouse immunized
with F9 teratocarcinoma stem cells (X-irradiated).
MC-631 (SSEA-3) hybridomas were produced by fusing
SP2/0 mouse myeloma cells with splenocytes from a Fisher rat immunized
with 4-8 cell stage mouse embryos.
MC-813-70 (SSEA-4) hybridomas were produced by
fusing SP2/0 myeloma cells with splenocytes from a BALB/c mouse
immunized with human embryonal carcinoma cell line 2102Ep.
TRA-1-60 and TRA-1-81 hybridomas were produced
by fusing SP2/0 myeloma cells with splenocytes from a BALB/c mouse
immunized with human embryonal carcinoma cell line 2102Ep.
Reactivities
and Isotype: MC-480 (SSEA-1): early mouse embryos, mouse embryonal
carcinoma cells, mouse embryonal stem cells, primordial germ cells;
IgM
MC-631 (SSEA-3) and MC813-70 (SSEA-4): early
mouse embryos, human teratocarcinoma stem cells, human embryonal
stem cells. MC-631, IgM; MC-813-70, IgG3
TRA-1-60 and TRA-1-81: human embryonal carcinoma
cell lines. IgM.
Applications:
Immunohistochemistry, Immunoprecipitation. Identification of human
embryonic stem cells. Characterization of embryonal stem cells of
human origin to distinguish them from those of murine origin.
References:
Solter, Knowles (1978) Monoclonal antibody defining a stage-specific
mouse embryonic antigen (SSEA-1). Proc. Natl. Acad. Sci. 75: 5565?5569.
Kannagi, Cochran, Ishigami, Hakomori, Andrews,
Knowles and Solter (1983) Stage-specific embryonic antigens (SSEA-3
and-4) are epitopes of a unique globo-series ganglioside isolated
from humanteratocarcinoma cells. EMBO 2(12): 2355?2361.
Shevinsky, L.H., Knowles, B.B., Damjanov, I.,
and Solter, D._(1982)_ A stage-specific embryonic antigen defined
by monoclonal antibody to murine embryos, expressed on mouse embryos
and human teratocarcinoma cells._ Cell 30: 697-705.
Andrews P.W. et al. (1984) Three monoclonal antibodies
defining distinct differentiation antigens associated with different
high molecular weight polypeptides on the surface of human embryonal
carcinoma cells. Hybridoma 3: 347-361.
Marrink J., Andrews P.W., van Brummen P.J., de
Jong H.J., Sleijfer D., Schraffordt-Koops H. and Oosterhuis J.W.
(1991). TRA-1-60: A new serum marker in patients with germ cell
tumors. Int. J. Cancer 49: 368-372.
Giwercman, A., Andrews, P.W., Jørgensen, N.,
Muller, J., Graem, N., Skakkebaek, N.E. (1993) Immunochemical expression
of embryonal marker TRA-1-60 in carcinoma in situ germ cells and
in testicular germ cell tumours. Cancer 72: 1308-1314.
Andrews, P.W., et al. (1996). Comparative analysis
of cell surface antigens expressed by cell lines derived from human
germ cell tumours. Int. J. Cancer 66: 806-816.
Gels, M.E., Marrink
J, Visser, P., Sleijfer, D.T., Droste
J.H.J., Hoekstra, H.J., Andrews, P.W.,
Koops, H.S. (1997). Importance of a new
tumour marker TRA-1-60 in the follow-up
of patients with clinical state I nonseminomatous
testicular germ cell tumours. Annals of
Surgical Oncology 4: 321-327.
Contact:
Meryle J. Melnicoff, Ph.D.
Director, Business Development The Wistar Institute
Phone: (215) 898-0049
melnicoff@wistar.org
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