Monoclonal Antibody A32 Against MEL-CAM/MUC18

Inventors:    Meenhard Herlyn

Tech ID:    HM-01RR

Description: Origin: Hybridoma cells producing MAb A32 were obtained after fusion of the murine myeloma cell line P3X63Ag8.653 with spleen cells derived from BALB/c mice which were immunized by i.p. injection of 1.5 x 107 human primary WM902B melanoma cells and boosted with the same treatment procedures 2 and 7 weeks later.

Cell surface melanoma-associated antigens can mediate cell-cell or cell-substrate adhesion, signal transduction, proteolysis, or immune recognition and play a key role in determining invasive and metastatic competence of the tumor cells. MAb A32 binds to endothelial and melanoma cells and detects a 113 Kd cell surface adhesion receptor of the immunoglobulin supergene family. The Mel-CAM/MUC18 antigen is a cell surface molecule involved in heterotypic adhesion. The ligand for Mel-CAM/MUC18 has not yet been identified. Its characterization will be important for endothelial cell adhesion during inflammation.

Isotype: IgG1

Applications: immunohistochemistry, western blot, endothelial cell identification (demonstrating strong staining of all endothelial cells), frozen tissue sections, melanomas.

Contact:

Meryle J. Melnicoff, Ph.D.
Director, Business Development The Wistar Institute
Phone: (215) 898-0049
melnicoff@wistar.org