Developmental Embryonic Mouse cDNA Libraries

Inventors: Barbara Knowles, Jay L. Rothestein and Davor Solter

Tech ID: KB-9210

Description: The molecular control of mammalian preimplantation and embryogenesis remains largely unexplored due in large part to the difficulty of obtaining sufficient quantities of suitably timed embryos for experimentation. Nevertheless, knowledge about the changes in gene expression that underlie preimplantation and embryogenesis is essential to understanding mammalian development.

cDNA libraries have been derived from unfertilized eggs, and two-cell, eight-cell, and blastocyst-stage mouse embryos. Additionally, two specialized subtractive libraries have been prepared from the 2-cell stage embryo.

Key Words: Research tool, directed evolution

Applications and Advantages: These cDNA libraries are a unique resource for scientists whose work necessitates the ability to identify and isolate genes that are expressed in the early mammalian embryo. Successful isolation of these genes can subsequently lead to identification of reagents for assays to detect agents capable of controlling cell growth and differentiation. Additionally, these genes, or antibodies to these genes, may provide a marker or act as a diagnostic agent for early pregnancy loss.

Intellectual Property Status: U.S. Patent 5,789,158 has been issued.

Licensing Opportunity: Wistar is seeking non-exclusive licenses for research use.

Contact:

Meryle J. Melnicoff
Director, Business Development
The Wistar Institute
3601 Spruce Street
Philadelphia, PA 19104
Phone: (215) 898-0049
Fax: (215) 573-2456
melnicoff@wistar.org

Last updated: Nov. 99