Anti-99Pst is derived from rabbits immunized with a recombinant BIN1 polypeptide fused to glutathione s-transferase. 99D-I are secreted by hybridomas derived from mice immunized with recombinant BIN1 polypeptide fused to glutathione s-transferase. Isotypes are IgG1 (E, G, H) and IgG2b(D, E).

The polyclonal antibody recognizes BIN1 and at least one additional BIN1-related protein. This antibody does not appear to recognize the murine BIN1 polypeptide and therefore appears to be human-specific. The monoclonal antibodies are specific for BIN1 but recognize proteins from human, murine, rat, and avian cells. The epitope recognized by 99F, 99G, 99H, and 99I has been mapped to a 10-residue region of BIN1. Two other epitopes mapping outside of this region are recognized by the three other antibodies.

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