Method for Solid-Phase Amplification of DNA Using Multiarrays
G. Rovera and S. Mukhopadhyay
Amplification of DNA is a common first step in nucleic acid diagnostics, particularly when testing for the presence or absence of an organism or a given nucleic acid sequence in a sample. For simplicity and sensitivity, DNA amplification assays have been developed wherein either the nucleic acid template or the primer is attached to a solid phase. These assays are particularly suitable for screening multiple samples in an automated system.
Wistar researchers have developed an improved assay for detecting the presence of a given test DNA in a sample, wherein the 5¹ and 3¹ primers for the test DNA are irreversibly bound to a solid phase and the DNA in the sample is reversibly bound to the solid phase. When these components are incubated under conditions that promote nucleic acid amplification, no signal is detectable unless the sample contains the test DNA. This assay may be useful for high throughput testing for pathogens (e.g. microorganisms or DNA viruses) or for detecting mutations in test samples, e.g. in multiarray systems.
This assay system has the potential for greater sensitivity than other solid phase DNA assays.
U.S. Patent No. 6,221,635.
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