How to Obtain Services

Illumina Next-Generation Sequencing

We require the following two sheets: NGS Sample Submission & Analysis Form (Excel file) and Bioinformatics Analysis agreement form, to be electronically filled out and emailed to Shashi Bala prior to each sample submissions. Next-Generation sequencing requires time and an understanding of the inherent complexities of sequencing. We recommend that each investigator takes time before submitting samples to discuss the project with one of our NGS specialists (genomics@wistar.org) in the facility. Consultation with Bioinformatics (bioinfo@wistar.org) is highly encouraged especially for new projects. Additional details of analysis rates are posted on the Bioinformatics website.

nanoString nCounter Analysis System

For questions about scheduling and cost, contact Celia Chang. All samples submitted for nanoString ncounter analysis must be accompanied by the Sample Form (Word file).

qPCR and primer design for gene expression validation, SNP Genotying analysis and C.bovis Detection  using ABI 7900 HT Fast Real-Time qPCR System

For questions about scheduling and cost, contact Celia Chang. All samples submitted for qPCR analysis must be accompanied by the Sample Form (Word file).

For C.bovis detection, please complete the C.Bovis Sample Submission Form and contact Ms. Tran Nguyen or genomics@wistar.org.

Capillary Sequencing and Microsatellite Analysis

Contact Ms. Tran Nguyen or genomics@wistar.org to schedule your sequencing. Please complete the DNA Sequencing  Sample_Submission Form (Excel File) or the Microsatellite STR HID Sample Submission Form. Ms. Nguyen can also be reached at 215-898-3991.

 

General Technical Support

If you need assistance in optimizing your assay conditions, please email Celia Chang, or, if urgent, call 215-898-3902. You should expect an answer the same day.

For large projects requiring planning and consultation, please contact the Scientific Director, Dr. Louise Showe, to schedule a meeting.