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Louise Showe, Ph.D.

Louise C. Showe, Ph.D.

Laboratory

The Showe Laboratory

Contact

215-898-3791
lshowe@wistar.org

Professor, Molecular & Cellular Oncogenesis Program

Associate Director, Center for Systems & Computational Biology

Scientific Director, Genomics Facility

Scientific Director, Bioinformatics Facility

About the Scientist

Showe’s research interests focus on using genomics-based approaches and big data to better understand disease processes with an emphasis on the immune system and cancer. Her laboratory has actively contributed to the development of genomics and bioinformatics at The Wistar Institute, implementing new methods for the generation and analysis of large and complex datasets.

Showe received her bachelor’s degree in biology from Wilkes College (PA) and her master’s in developmental biology from Temple University. After obtaining her Ph.D. in biology from the University of Pennsylvania, she moved to Europe to conduct her postdoctoral studies at the Biozentrum der Universitat, Basel, Switzerland. Showe joined The Wistar Institute in 1983 as an assistant professor and became a full professor in 2007.

View Publications

The Showe Laboratory

The Showe laboratory applies genomics approaches to answer complex questions in the context of immunology and cancer with the ultimate goal of developing novel diagnostic tools and markers of therapy response and disease relapse. Through an extensive network of collaborations with other laboratories at Wistar and several other institutions, the lab focuses on a diversified group of diseases, including lung cancer and cutaneous T cell lymphoma, HIV and influenza infections, and multiple sclerosis. The lab’s diverse interests are shown by a long-term collaboration with the University of Fairbanks to study how hibernating black bears modify gene expression in various tissues during hibernation.

Staff

Senior Staff Scientist

Kiran Gummireddy, Ph.D.

Wistar Scientist

Andrei Kossenkov, Ph.D.

Associate Wistar Scientists

Shashi Bala, Ph.D.
Rehman Quereshi, Ph.D.
Jayamanna Wickramasinghe, Ph.D.
Harsh Dweep, Ph.D.
Hidecki Tanizawa,Ph.D.

Wistar Research Assistants

Rajrupa S. Majumdar
Tran Nguyen
Sandy Widura

System Developer & Administrator

Wen-Hwai Horng

Research

Biomarkers for Early diagnosis of Non-Small Cell Lung Cancer

The Showe lab has been a pioneer in the development of cancer biomarkers from blood. In 2009, they published novel findings showing how mononuclear white blood cells (PBMC) from lung cancer patients contain a 29-gene, tumor-relevant gene signature that accurately predicts whether a lung nodule detected by CT scan is benign or malignant. They also demonstrated that information in blood gene expression patterns could predict patient survival and inform further treatments. In moving these studies forward to clinical application the lab has adopted a simplified and standardized sample collection and moved their assay to the Nanostring platform, a variation of the DNA microarray technology. The technology has been licensed and is in development for commercialization.

Ongoing effort: As part of the NCI Early Detection Network the lab will developing new methods to detect and diagnose cancers early, when they are more easily and successfully treated, and develop new methods to predict outcomes after treatment. This is a collaborative study with investigators at The University of Pennsylvania, NYU, Temple University, The Helen Graham Cancer Center, Fox Chase Cancer Center and Meridian Health.

Gene Expression in Cutaneous T-Cell Lymphoma

Cutaneous T-cell lymphomas (CTCL) are a heterogeneous group of non-Hodgkin lymphomas with characteristics of “skin-homing” T lymphocytes. The most common forms of CTCL are the skin associated Mycosis Fungoides (MF) and a more aggressive leukemic form,Sezary Syndrome (SS),.. SS has been the focus of the laboratory studies. Early interest in this cancer was based on the observation that patients were severely deficient in the production of interleukin 12, a cytokine originally identified and characterized at The Wistar Institute, and on availability of a unique collection of patients being treated at the University of Pennsylvania Department of Dermatology. Based on initial studies, Showe was awarded one of the first National Cancer Institute Director’s Challenge grants to develop molecular diagnostics for CTCL using microarray platforms. This study led to the identification of a small number of genes that could detect SS by microarray or Q-rtPCR and identified some new specific cellular defects and a signature of poor prognosis that was independent of stage or circulating malignant cell numbers.

Ongoing studies: in collaboration with clinicians at the University of Pennsylvania (Alain Rook and Ellen Kim), Columbia University (Susan Bates) and NCI (Richard Piekarz), present work focuses on understanding mechanisms of therapeutic response both in vitro and in vivo to combined treatments such as toll-receptor agonists and interferon as well recently FDA-approved treatments with histone deacetylase inhibitors. Combining data from gene and microRNA expression, produced using microarrays and next-generation sequencing, the lab is defining parameters that distinguish responders from non-responders and markers of residual disease that can be monitored to detectrecurrences before they are clinically evident. Using single cell analysis they are probing malignant cell heterogeneity and therapeutic response.  A recent award from GSK focuses on the CTCL work and potential cancer therapies.

Functional Genomics of HIV

In a long term collaboration with the lab of Luis J. Montaner, D.V.M., D.Phil., at Wistar, the Showe lab applies functional genomic approaches to understand mechanisms of immune evasion in HIV infections and explore new ways to manage current HIV-1 infections, including the possibility of using immune-mediated control of virus infection upon interrupting drug therapy. Additional studies have focused on developing biomarkers to assess secondary infections, such as Tuberculosis, in the presence of an active HIV infection  She is a Co-Investigator on the BEAT-­HIV Delaney Collaboratory grant to continue these studies.

Gene Expression in Multiple Sclerosis

In collaboration with Cris Constantinescu, M.D., Ph.D., at The University of Nottingham, UK, the Showe laboratory is examining gene expression profiles in blood samples from multiple sclerosis patients involved in clinical trials with immuno-modulatory drugs. The aim is to determine whether it is possible to identify gene expression patterns that correlate with responsiveness and non-responsiveness to therapy.

Gene Expression and Vaccines

Studies in collaboration with Hildegund C.J. Ertl, M.D., at Wistar, have examined gene expression changes in young and aged populations as a function of flu vaccination to try to understand the poorer responses to vaccinations in general in the elderly population in particular.  In addition, potential effects of race and timing of repeated vaccination on response were examined. Additional studies using mouse models have explored protocols that increase vaccine response with the potential benefit resof improving vaccine efficacy in the growing elderly population.