Genea

Adenovirus

Lentivirus

Footnotes

bFGF

bFGF-S (18 kDa)

J.Bennet, Penn 

Steven Kazianis

bFGF-L (18, 22, 23, 24 kDa)

 J.Bennet, Penn,

shFGFR4

Broad Institute(x3)

shFGFR1

Broad Institute (x5)

VEGF

VEGF (VEGF-A)-121

Mark Nesbit

VEGF-C

Zhao-Jun Liu

VEGF-D

Zhao-Jun  Liu

PLGF

Peter Carmeliet

VEGF-Trap (R1/R2)

Regeneron

FLT-1 DN

Helmut Schaider

KDR

Helmut Schaider

Angiopoeitin

Angiopoietin (Ang)-1

Regeneron

1*

Angiopoietin-2

Regeneron

1

Tie2-Fc

Regeneron

1

PDGF/SCF

PDGF-A

Satya

PDGF-B

Mark Nesbit

SCF

Satya/Taka

(mb and secreted forms) Steven Kazianis

PDGFR-b-Fc

Regeneron

1

c-kit (retrovirus)

Satya

in progress

Sh-c-kit

Broad Institute (5)

shPDGFa

Broad Institute (5)

shPDGFß

Broad Institute (5)

TGF-b

TGF-ß1

 P. Robbins, Porcine

Steven Kazianis(human version)

2**

TGF-ß3

Tim Crombleholme

2

TGF-ß3-GFP

Tim Crombleholme

2

shTGF-ß1

Broad Institute (5)

BMP-7

Renny Franceschi

2

shTGFBRII

Broad Institute (13)

Endothelins

ET-3 (EDN3)

Satya/Takahashi

Steven Kazianis

EDNRB

Steven Kazianis

Midkine

Pleiotrophin

Satyamoorthy

HGF

HGF/SF

Jim Wilson

Steven Kazianis (in progress)

2

Shc-met

Broad Institute (13)

Insulin

IGF-1

Satya

IGF-2

Trish Brafford

shIGF1R

Broad Institute (12)

shIGF2R

Broad Institute (5)

EGF and related

TGF-a

TrishBrafford

EGFR

Dorothee Herlyn

2

EGFRvIII (mut)

Dorothee Herlyn

2

shEGFR (shERBBB1)

Broad Institute (1)

shERBB3

Broad Institute (5)

shERBB4

Broad Institute (5)

Chemokines

 IL-8

Masahiro Oka

AS IL-8

Oka/Mark

MCP-1

Mark Nesbit

shCXCR2 (IL8RB)

Broad Institute

Cytokines

mIL-10

Paul Robbins

2

IL-2

Frank Graham

2

IL-12.1

Frank Graham

2

Integrins

DNM b3

Zhao-Jun Liu

ß3 integrin

Clayton Buck

a2 integrin

C. Buck

a5 integrin

C. Buck

DNM-b1

C. Buck

shRNAa6 integrin

Niko Haass, Broad Institute (5)

Shbeta33

Broad Institute (5)

Connexin

shRNA Cx 43

Steven Kazianis (1)

Matricellular Proteins

CCN3

Robert Baxter

Mizuho Fukunaga

1

shRNACCN3

Mizuho Fukunaga

SPARC

Hynda Kleinman

shSPARC

Mizuho Fukunaga

shOsteopontin

Mizuho fukunaga

Shtenascin C

Mizuho Fukunaga

CAM/Other

Mel-CAM

Satya

shMel-CAM

Broad Institute

L1-CAM

Trish Brafford

L1-CAM, DNM, cytoplasmic domain deleted

Trish Brafford

shRNADDR1

Mizuho Fukunaga

Cadherins/WNT

E-cadherin

Mei-Yu Hsu

N-cadherin

Gary Li

Cad.-DNM, (cytoplasmic domain of N-cadherin deleted)

Gary Li

N-cad-DNM (extracellular domain deleted)

Gary Li

Desmoglein 3, DNM

Hanakawa

2

mDesmoglein3

Kyongun Yoon

2

Wnt3a

Victor Engelhart

Steven Kazianis

2

ß-catenin, dom. Active

Tom Force

2

ß-catenin wt

Jun Liu

Wnt5a

Steven Kazianis

Sh-ß-catenin

Broad Institute (4)

shN-cadherin

Broad Institute (5)

EGP

GA733

Dorothee Herlyn

2

Ephrin/Eph

Ephrin A1-FC

Regeneron

Soluble ephrin1

Ephrin A2-Fc

Regeneron

1

EphA2

Chelsea Pinnix

EphAs DNM

Chelsea Pinnix

Gene

Adenovirus

Lentivirus

Notes

Enzymes and Inhibitors

MMP-9 (activated)

Andrew Baker

2

muPA

Peter Carmeliet

2

uPA

Peter Carmeliet

2

tPA

Peter Carmeliet

2

PAI-1

Peter Carmeliet

2

AS uPAR

Justi Rao

2

Cell cycle

p16 (CDKN2A)

Satya

shRNA CDKN2A (INK4A p16)

Steven Kazianis (1)

shRNA CDKN2A (p14 ARF)

Steven Kazianis (1)

p21 WAF1

Wafik El-Deiry

2

shcyclinD1

Broad Institute (8)

AS cyclin D1

Ed Sauter

p53

Wafik El-Deiry

2

shp53

Maria Soengas (2)

2

MAPK pathway

n-ras

Joe Nevins

Steven Kazianis

2

n-ras, dom. Active codon 61

Joe Nevins

Steven Kazianis

2

H-ras

Maria Soengas

2

BRAF

Steven Kazianis

BRAFV600E

Steven Kazianis

shRNABRAFV600E

David Tuveson (2)

2

shRNABRAFwt and V600E

David Tuveson (2)

2

shRNABRAF wt

David Tuveson

2

shRNA RAF-1

David Tuveson

2

Apoptosis

shRNA BCL2

Maria Soengas

2

shBAD

Steven Kazianis (5)

PI3K pathway

p85 DNM PI3K

Mas. Oka

P110 constit. Act. PI3K

Mas. Oka

Myr-Akt, constitut. Act. AKT

Mas. Oka

2

DN AKT/A4

Jin Chen

2

PDPK1

Steven Kazianis

shPTEN

Broad Institute (8)

Rho/Rac

Cdc42 wt

Barry Ziober

2

Cdc42 N17 DNM

Barry Ziober

2

Rac-1 wt

Barry Ziober

2

Rac-1 DNM

Barry Ziober

2

Rho A wt

Barry Ziober

2

RhoA-N19 DNM

Barry Ziober

2

Other Oncogenes

c-myc

Wafik El-Deiry

HPV 16 E6

Satya

HPV 16 E7

Stephen Eck

shMDM2

Broad Institute (5)

shMDM4

Broad Institute  (5)

Control vectors

Lac Z

Vector Core

Steven Kazianis

GFP

Vector Core

Steven Kazianis

DL7001

Vector Core

RR5CMV

Peter Carmeliet

Fc

Regeneron

1

RedFP

Steven Kazianis

YFP

Steven Kazianis

pECFP

Steven Kazianis

H1UG (w/GFP)

Eric Brown

2

FG12

Maria Soengas

2

pL6 empty

Steven Kazianis

pL4 empty

Steven Kazianis

pL4 GUS

Steven Kazianis

Helpers

pHCMV-G

pMDLg/pRRE

pRSVrev

pLP/VSVG

Invitrogen

pMD2.G

psPAX2

pLP1

Invitrogen

pLP2

Invitrogen

Other

MITF

Satya

FGF-BP (HBP-17)

Mark Nesbit

Gem/Kir

Satya

TVA950 (chicken)

Trish Brafford

hTERT

Steven Kazianis

1

PP2A

Src wt

Rena Finko

Tropomodulin wt

Clayton Buck

Tropomodulin AS

Clayton Buck

Skp wt

Dieter Wolf

Skp DNM

Dieter Wolf

shp38alpha

John Lee

shDDR1

Mizu Fukunaga

BMI1

Steven Kazianis

shSTAT3

Broad Institute

Notch

Notch 1 cyto.domain act. (NIC)

Jun Liu

Jun Liu (with GFP)

Notch1 wt

Jun Liu

HES1

Jun Liu

DLL4

Jun Liu

DLL4-Fc

Jun Liu

RBPJkappa DN

Tasuko Honjo

2

NFkB

IKBKB

Yingling Hu

2

NFKbiA

Yingling Hu

2

RelA p65

Yingling Hu

2

PKC

PKCA wt

Mas. Oka

2

PKCA DN

Mas. Oka

2

PKCß1 DN

Mas Oka

2

PKCß1 wt

Mas. Oka

2

PKCdelta wt

Mas. Oka

2

PKCdelta DN

Mas. Oka

2

PKCepsilon wt

Mas. Oka

2

PKCepsilon DN

Mas. Oka

2

PKCzeta wr

Mas. Oka

2