Histotechnology Facility
Overview
The Histotechnology Shared Resource provides services for fixing, processing and paraffin or OCT-embedding of all types of tissues for light microscopy (e.g. routine stains, immunohistochemistry or in situ hybridization). The Facility staff performs routine hematoxylin, eosin staining, immunochemistry and FISH staining, as well as specialized staining and slide preparation for immunohistochemistry and in situ hybridization. Frozen sectioning is also available, including consultation regarding freezing and fixing techniques to optimize experimental results.
Services
- Processing, embedding, sectioning, and histochemical staining of specimens
- Paraffin and frozen sectioning are routinely available for light microscopy and in situ hybridization
- Tissue preparation for DNA/RNA extraction
- Various histochemical staining procedures are performed, including those required for neuropathology
- Special procedures and staining protocols
- Consultation regarding freezing and fixing techniques and immunohistochemistry staining techniques
Equipment
- Thermo cryostar NX50
- Thermo Excelsior AS tissue processor
- Dakewe auto stainer
- Dakewe coverslipping machine
- TissueTek embedding center
- Thermo HM355S microtome
- Biocare Intellipath IHC stainer
- Dakewei Cryostat CT520
- Tissue Processor HP300
Pricing
For pricing information, visit iLab or contact the managing director.
Selected Publications
Na+/H+-exchanger 1 enhances antitumor activity of engineered NK-92 natural killer cells
Gong Y-Y, Shao H, Li Y, Brafford P, Stine ZE, Sun J, Felsher DW, Orange JS, Albelda SM, Dang CV. 2022. Na+/H+-exchanger 1 enhances antitumor activity of engineered NK-92 natural killer cells. Cancer Res Commun, 2:842-856. doi: 10.1158/2767-9764.
An African-Specific Variant of TP53 Reveals PADI4 as a Regulator of p53-Mediated Tumor Suppression
Indeglia A, Leung JC, Miller SA, Leu JI-J, Dougherty JF, Clarke NL, Kirven NA, Shao C, Ke L, Lovell S, Barnoud T, Lu DY, Lin C, Kannan T, Battaile KP, Yang THL, Batista Oliva I, Claiborne DT, Vogel P, Liu L, Liu Q, Nefedova Y, Cassel J, Auslander N, Kossenkov AV, Karanicolas J, Murphy ME. 2023. Cancer Discov, 13:1696-1719. doi: 10.1158/2159-8290.
Acetate acts as a metabolic immunomodulator by bolstering T-cell effector function and potentiating antitumor immunity in breast cancer
Miller KD, O’Connor S, Pniewski KA, Kannan T, Acosta R, Mirji G, Papp S, Hulse M, Mukha D, Hlavaty SI, Salcido KN, Bertolazzi F, Srikanth YVV, Zhao S, Wellen KE, Shinde RS, Claiborne DT, Kossenkov A, Salvino JM, Schug ZT. 2023. Nat Cancer, 4:1491-1507. doi: 10.1038/s43018-023-00636-6
Decitabine disrupts EBV genomic epiallele DNA methylation patterns around CTCF binding sites to increase chromatin accessibility and lytic transcription in gastric cancer
Preston-Alp S, Caruso LB, Su C, Keith K, Soldan SS, Maestri D, Madzo J, Kossenkov A, Napoletani G, Gewurz B, Lieberman PM, Tempera I. 2023. mBio, 14:e0039623. doi: 10.1128/mbio.00396-23
Dasatinib Resensitizes MAPK Inhibitor Efficacy in Standard-of-Care Relapsed Melanomas
Rebecca VW, Xiao M, Kossenkov A, Godok T, Brown GS, Fingerman D, Alicea GM, Wei M, Ji H, Bravo J, Chen Y, Fane ME, Villanueva J, Nathanson K, Liu Q, Gopal YNV, Davies MA, Herlyn M. 2023. bioRxiv, doi: 10.1101/2023.01.20.524923
The Ashkenazi-Centric G334R Variant of TP53 is Severely Impaired for Transactivation but Retains Tumor Suppressor Function in a Mouse Model
Stieg DC, Casey K, Karisetty BC, Leu JI-J, Larkin F, Vogel P, Madzo J, Murphy ME. 2024. Mol Cell Biol, 44:607-621. doi: 10.1080/10985549.2024.2421885.
The Wistar Institute
Histotechnology Facility
Room 258
3601 Spruce Street
Philadelphia, PA 19104
215-898-0027
Daniel Claiborne, Ph.D.
Scientific Director of the Histotechnology Facility
Fangping Chen, HT (ASCP)
Managing Director
9:00 a.m. – 5:00 p.m.
Monday-Friday
[Contact the facility to make arrangements for sample submission and pickup.]