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Genomics/Microarray Facility
Introduction
The Wistar Institute
Rooms G15, G54 and G58
3601 Spruce Street
Philadelphia, PA 19104
Tel: 215-898-3902
Fax: 215-898-4521
arraysupport@wistar.org
Facility Hours: 9:00 AM - 5:00 PM, Monday through Friday
Louise C. Showe, Ph.D.
Scientific Director
215-898-3791
Showe laboratory homepage
Array facility homepage
Celia Chang, Ph.D.
Facility Director
Shere Billouin
Research Assistant II
Genomics Generalist
Calen Nichols
Wistar Research Assistant
Solexa Genome Analyzer
Tran Nguyen
Research Assistant I
ABI DNA Sequencing and Microsatellite Analysis
Wen Hwai Horng, M.A., M.S.
Wistar Research Assistant
System Developer & Administrator, Array Analysis
Michael K. Showe, Ph.D.
Senior Staff Scientist
Data Analysis, Quality Control
Ellena Nikonova, M.D.
Staff Scientist
ABI Taqman assays and TLDAs
Introduction
The Wistar Genomics Facility serves as a hub for consultation and scientific interactions relating to nucleic-acid based methods and provides expertise and support to insure the best possible use of the emerging nucleic-acid technologies. In addition to consultation and collaboration with Wistar Cancer Center members, the Facility provides the following products and services to the greater scientific community.
Illumina Bead Array Platforms:
( http://www.illumina.com/pages.ilmn?ID=208 )
Solexa Genome Analyzer: ( http://www.illumina.com/pages.ilmn?ID=250 )
All sample preparations for GAII analysis are provided by the facility. User prepared samples are also accepted. Please email the GAII Submission Form and consult with Calen Nichols nichols@wistar.org for sample requirements, the quality control issues that need to be addressed in sample preparations,and the projected schedule for sample processing. A short description of the project should be provided with the samples.
Custom cDNA arrays can be produced at the request of investigator and by consultation with the Facility Director.
Capillary DNA sequencing and Microsatellite Analysis on ABI 3130xl sequencer.
miRNA profiling using ABI TaqMan low density arrays.
High through-put RealTime PCR using the ABI-7900HT Fast Real-Time PCR System with 384 well microfluidic PCR card capabilities. (https://products.appliedbiosystems.com/ab/en/US/adirect/ab
?cmd=catNavigate2&catID=600694 )
- Q-PCR and primer design
- Microarray validation
- Array bioinformatics
- Protocols
*The establishment of this facility was supported in part by an NCI Cancer Center Support Grant and equipment grants from the Commonwealth of Pennsylvania, The Pew Charitable Trusts and the National Cancer Institute.
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