| Mouse
Genetics Facility
The Wistar Institute
Room B86
3601 Spruce Street
Philadelphia, PA 19104
Tel: 215-495-6804
Fax: 215-495-6809
Facility Hours: 9:00 a.m. - 5:00 p.m., Monday through Friday
Joe Kissil, Ph.D.
Scientific Director
Ping Jiang, M.D.
Facility Director
215-495-6800
Yifang Huang, M.S.
Research Assistant II
215-495-6804
Chang Shan, B.S.
Research Assistant II
215-495-6804
Introduction
Mission of the Facility
To foster scientific discoveries, innovations,
and achievements by delivering advanced technology and excellent
support to investigators at Wistar and other biomedical institutions
around the nation.
Overview of the Facility
Services and Technologies
- Production of transgenic mice by pronuclear injections of transgene constructs
- Production of knockout/knockin mice by injecting gene-targeted ES cells into blastocysts
- Generation of gene-targeted embryonic stem cell clones by homologous recombination
- Embryonic stem cell technology (cultivation, propagation and genetic engineering)
- Rederivation by embryo transfer
- Embryo cryopreservation and rederivation from cryopreserved embryos
Services and Technologies in development: lentivirus-based transgenesis, tetraploid complementation, and optimization of human ES cell cultivation, propagation and differentiation.
The Facility
Facility laboratory. Our newly rebuilt Facility occupies over 1,700 sf of laboratory space consisting of 3 functional units: a micromanipulation and mouse reproduction unit, a cell culture unit, and a molecular biology unit—each is supplied with state-of-the-art equipment. The production of both of transgenic and knockout/knockin mice as well as the embryo transfer are performed in the Facility’s micromanipulation and mouse reproduction unit, located in a barrier area of Wistar’s Animal Facility. The Animal Facility is accredited by the Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC), a private, nonprofit organization that promotes the humane treatment of animals in science through voluntary accreditation and assessment programs.
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