Mouse Genetics Facility

Ping Jiang, M.D., Director
Room B86
3601 Spruce Street
Philadelphia PA 19104
Lab: (215) 495-6804
Office: (215) 495-6800
Fax : (215) 495- 6809

Introduction

Mission of the Facility

To foster scientific discoveries, innovations, and achievements by delivering advanced technology and excellent support to investigators at Wistar and other biomedical institutions around the nation.

Overview of the Facility

The Technologies

• The generation of transgenic mice by pronuclear injections of transgene constructs
• The generation of knockout/knockin mice by injecting gene-targeted ES cells into blastocysts
• The generation of gene-targeted embryonic stem cell clones by homologous recombination

Technologies in development: lentivirus-based transgenesis, siRNA knockdown mice, reprogramming and cloning by nuclear transfer and tetraploid complementation.

The Facility

Our newly rebuilt Facility consists of 3 functional units: a micromanipulation and mouse reproduction unit, a cell culture unit, and a molecular biology unit—each is supplied with state-of-the-art equipment. The Facility occupies a barrier animal facility within the Institute that is well recognized for its long history of pathogen-free operation. It is accredited by the Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC), a private, nonprofit organization that promotes the humane treatment of animals in science through voluntary accreditation and assessment programs.

The Services

The Facility offers a range of service levels—Essential service, Full service, and Optional service—which allows investigators to choose the technologies and tools that best suit their research. The Facility’s highly trained scientific staff provides professional assistance to investigators on a fee-for-service or collaboration basis. For a detailed description of the support we provide, click the Services link on the right.