Melanoma Cell Lines

Melanoma cell lines grouped for BRAF/-N-ras/PTEN/CDK4 mutations

Due to the high demand for our cell lines, we are pleased to partner with Rockland Immunochemicals Inc. for distribution.

Please contact Rockland directly about acquiring our melanoma cell lines by clicking here or contact Rockland customer service for help with lines not found yet on their website.

For help selecting lines, see the mutational chart below or search using Rockland's website.  For any questions, concerns, help or any other issues, please email Trish Brafford at Wistar or contact customer service at Rockland.

Genomic DNA from samples were analyzed for mutations by the nucleotide extension assay using the iPlex platform (Sequenom, Inc, San Diego, CA) (Thomas, Baker et al. 2007) by Kate Nathanson, University of Pennsylvania (manuscript in preparation).  PTEN status reported is a compilation of aCGH data generated with the 250k Styl SNP Affymetrix Array (Nathanson) and the published data by Lin WM et.al. (Cancer Res. 2008 Feb 1;68(3):664-73)  PTEN data is yellow is data that conflicts from the various methods. Cell lines lumped together with the same color are different samples from the same patient.

Cell lines Stage BRAF PTEN N-ras c-KIT CDK4 OTHER homozygous loss high copy gain growth
1205 Lu Xenograft MET to WM793 V600E Mu/Hem Del WT WT K22Q GRM3, MAP3K5, TERT CDKN2A CDKN2B KRAS 1:10 <1 WK
451Lu Xenograft MET to WM164 V600E ND WT WT WT TP53, CDKN2A, ROS1, EZH2   CCND1 FGF3 FGF4 MITF 1:10 <1 WK
451lLu BR BRAF/MEK Resistant V600E ND WT WT WT CDKN2A, EZH2, HRAS, MAP2K2, TERT, TP53 BRCA2 DC MAP3K5 PPP6C RB1 ROS1 SMAD4 BRAF CCND1 FGF3 MITF SOX10  
WM1026 LN MET V600E ND WT WT WT CDK4, PREX2, TERT ARID1B ATM   1:3 1.5 wk
WM115 VGP V600D Hem Del WT WT WT GRM3, TERT CDNK2A CDKN2B   1:3 1 wk
WM165-1 MET V600D Hem Del WT WT WT GRIN2A, GRM3, TERT CDKN2B   1:4 1 wk
WM239A MET V600D Hem Del WT WT WT GRM3, TERT CDNK2A CDKN2B   1:4 <1wk
WM266-4 MET V600D Hem Del WT WT WT GRM3, TERT CDNK2A CDKN2B   1:3 1 wk
WM1158 MET V600E V343E/Hem Del WT WT WT CDKN2A, MLH1, PTPRD, TERT     1:6 <1wk
WM1232 LN MET V600E Hem Del WT WT WT CDKN2A, ALK, EZH2, TERT, TP53   MITF 1:3 1 wk
WM1341D VGP V600R WT WT WT WT TERT CDNK2A CDKN2B   1:3 1.5 wk
WM1361A VGP WT ND Q61R HOM WT WT BAP1, GRIN2A, IDH2, PRDM2, PTCH1, PTPRD, TERT CDNK2A CDKN2B MDM2 1:4 1 WK
WM1366 VGP WT WT Q61L WT WT CDKN2A, DCC, DDX3X, FGFR2. GRIN2A, KDR, MAP3K9, NF1, PTPRD, RAC1, TERT     1:10 6 days
WM1552C RGP V600E Hem Del WT WT WT TP53 CDNK2A CDKN2B BAP1    
WM164 MET V600E WT WT WT WT TP53, CDKN2A, EZH2, TERT   CCND1 FGF3 FGF4 MITF 1:10 <1 WK
WM1716 MET V600E Hom del WT WT WT ARID2 ERBB4 FLT3 MAP3K5 PREX2 PTPRK TERT CDNK2A CDKN2B    
WM1727A MET V600E WT WT WT WT ATM, GRIN2A, PPP6C, PREX2, PTPRK, CDKN2A, MAP2K2 Y134H, TERT     1:3 4 days
WM1789 UNKNOWN K601E HOM, S363F WT WT WT WT MAP2K2 E207R, BRCA1, DDX3X, GRIN2A, PDGFRA, PIK3CA, ROS1, SETD2, SMARCCA4, TERT, TRRAP     1:3 6 days
WM1791C MET WT ND WT (KRAS T50I, E62K) WT K22Q RAC1, KRAS, BAP1, KDR, PDGFRA, PREX2, TERT, TRRAP CDNK2A CDKN2B   1:6 4 days
WM1799 MET V600E HOM Hem Del/ Hom Del WT WT WT ARID2, PTCH1, TERT, VAV3 CDKN2A FGF4 1:10 <1 WK
WM1819 UNKNOWN V600E ND WT WT WT TRRAP, TP53, INSRR, KDR, TERT     1:6 5 days
WM1852 MET V600E WT WT WT WT BAP1 ERBB4 CDKN2A    
WM1862 RGP V600E ND WT WT WT ALK NTRK1 CASP8 CDKN2A CDKN2B CCND1 FGF3 FGF4 NOTCH2 1:4 <1wk
WM1960 MET WT WT Q61K WT WT ARID2 CDKN2A GRM3 MLH1 PIK3CB PREX2 RAC1 TERT     1:3 2 wks
WM2013 MET WT ND Q61K WT WT ARID2, BRCA1, CDKN2A, GRM3, MLH1, PIK3CB, PREX2, RAC1, TERT     1:4 6 days
WM3060 MET WT WT Q61K WT WT CDKN2A, MLH1, RAC1, ARID2, BRCA1, GRM3, MLH1, PIK3CB, PREX2, TERT     1:3 5 days
WM1963 unknown N581S WT A146T WT WT ARID2, BRCA1,CTNNB1, KDR, NF1, PTPRD, TERT, WT1, TP53, KDR, MAP2K1 HOM,     1:5 1 wk
WM1985 UNKNOWN V600E Hom del WT WT WT TP53, TERT AKT1 CDKN2B   1:6 1 wk
WM2032 LN MET WT ND Q61R WT WT ARID2, TERT CDNK2A CDKN2B ATM MMP8   1:4 1 wk
WM3000 MET WT ND Q61R WT WT   CDNK2A CDKN2B ATM MMP8   1:6 1 wk
WM2044 MET V600E ND WT WT WT TP53, ALK, TERT   MITF 1:6 1 wk
WM209 MET WT ND G12A WT WT EGFR, NOTCH1, PREX2, RAC1, TERT APC CDNK2A   1:6 4 days
WM2090 MET V600E ND WT WT WT CDKN2A, DDDX3X, IDH1, TERT, WT1     1:6 5 days
WM262 MET V600E ND WT WT WT TERT CDKN2A   1:6 <1 wk
WM278 VGP V600E Hem Del WT WT WT ROS1, GRIN2A, RHOT1, ROS1 CDKN2A    
WM3061 MET WT WT G13R WT WT       1:3 3 wks
WM3211 VGP WT ND WT L576P WT CDKN2A, ARID1A, TP53, BRCA2, TERT DCC SMAD4   1:3 <1 wk
WM3246 MET WT (K80_R84 DEL) WT WT WT CDKN2A DCC EGFR SRP19 TP53 TERT GNA11   1:4 1 wk
WM3248 unknown V600E HOM K144X HOM WT WT WT APC, TERT CDNK2A CDKN2B   1:4 <1 wk
WM3268 VGP WT   Q61K WT WT ARID1A, ARID1B, TERT CDNK2A CDKN2B   1:4 1.5 wk
WM3282 VGP V600K HOM ND WT WT WT DCC, ERBB4, NTRK1, TP53, EGFR, ERBB2, FGF4, GRM3, KDR, KIT, NTRK1, PDGFRB, PREX2, PTPRK, TERT CDNK2A CDKN2B   1:3 1 wk
WM3301 MET V600E Hom del WT WT WT EZH2 PREX2 TERT CDNK2A CDKN2B   1:3 2 wks
WM3311 MET WT ND WT WT WT GRIN2A, PRDM1, TERT CDKN2A NOTCH2 1:3 1 wk
WM3406 MET WT MU Q61k WT WT AKT1 ARID1A GRIN2A HRAS TERT CDNK2A CDKN2B    
WM3438 MET WT ND WT WT WT DCC, FGFR4, GRIN2A, TERT CDNK2A CDKN2B MITF 1:3 2 wks
WM3456 MET WT WT Q61K WT WT ALK ARID2 DCC PREX2 ROS1 TACC1 CDNK2A CDKN2B CCND1 1:4 2 wks
WM3482 MET V600E HOM ND WT WT WT CDKN2A, SETD2, TERT     1:4 2 wks
WM3506 MET WT WT Q61R WT WT ARID1A EIF2AK3 GRM3 INSRR PREX2 ROS1 STK24 TACC1 TERT CDNK2A CDKN2B   1:4 1 wk
WM3533 LN MET V600E ND WT WT WT ARID2, IDH1, TERT PTPRK STK11   1:4 2wks
WM3540 LN MET V600E ND WT WT D97H TP53, FGFR2, ROS1   MITF 1:6 1 wk
WM3618F LN MET OF UVEAL PRIMARY WT ND WT WT WT GNAQ, BAP1, CTNNB1      
WM3772F MET OF UVEAL PRIMARY WT ND WT WT WT GNAQ, BAP1 CDKN2A PRDM1 PTPRK MYC PREX2 SNX31 1:4 1 wk
WM3619 MET WT ND Q61R HOM WT WT ROS1, ERBB4, INSRR, PREX2, ROS1, SOX10, TERT CDNK2A CDKN2B FGFR2 IDH2 1:3 1 wk
WM3622 MET WT F337S WT WT WT ABL1 ALK ARID1A DCC ERBB4 GRIN2A GRM3 MAP3K5 NF1 NOTCH2 PREX2 ROS1 TP53 VAV3 TERT CDNK2A CDKN2B PTPRD   1:4 1.5 wk
WM3623 LN MET WT ND Q61K WT WT ARID2, PRDM2, PREX1, PREX2, PTPRD, TERT     1:6 1 wk
WM3629 LN MET D549G het ND G12D hom WT WT   CDNK2A CDKN2B PTPRD CDK4 MDM2 1:6 1 wk
WM3630 MET V600E ND WT WT C70T TP53, PTCH1, TERT, TP53 BRCA2 RB1 GNAS 1:4 1 wk
WM3670 LN MET G469E WT G12D WT WT ALK APC ATM DCC DDX3X ERBB2 ERBB4 FGFR2 FLT3 MET MLH1 PREX2 PTPRD SOX10 CDKN2A   1:3 1 wk
WM3681 LI MET WT ND WT WT WT       1:6 1 WK
WM3682 LN MET WT ND Q61L WT WT       1:6 1 wk
WM3702 CUT MET WT ND Q61L WT WT DCC, EGFR, ERBB4, FBXW7, GRIN2A, MET, PREX2, TERT AKT1 APC CDKN2A CDKN2B TERT   1:4 1 WK
WM3704 MET V600E R130fs HOM WT WT R24H CDK6, FGFR2, INSRR, MITF, STK24, TACC1, TERT, TP53 CDNK2A CDKN2B   1:6 1wk
WM373 MET V600E WT WT WT WT ARID1A, EGFR, ERBB4, NF1, TERT CDK6 DYNC11 MET TRRAP    
WM3734 BRAIN MET V600E HOM DEL WT WT WT   CDNK2A CDKN2B NRAS MITF 1:6 1 wk
WM3743 PRIMARY, POST CHEMO   R15X HOM WT WT WT ARID2, CDKN2A, DCC, TP53, CXCR4, MET, NTRK1, TERT, TP53     1:5 2 wk
WM3749 MET V600E Hom del WT WT WT DCC AKT1 ARID1B CDKN2A CDKN2B   1:3 2 wks
WM3755 MET V600E HOM ND WT WT WT EZH2, GRM3, KDR CDNK2A CDKN2B   1:4 < 1 wk
WM3758 MET WT ND Q61L WT WT BRCA1 DCC EGFR FBXW7 GRIN2A MET PREX2 PTPRK TERT CDNK2A CDKN2B APC   1:13 1 wk
WM3792 MET V600K601del HOM DEL WT WT WT ARID1A, PPP6C ARID1B CDKN2A PRDM1 PTPRK   1:6 10 days
WM3854 LN MET WT   Q61L WT WT ARID1B, DCC, MET, RHOT1, TERT CDNK2A CDKN2B PTPRD CCND1 FGF3 FGF4 MMP8 NOTCH2 1:4 1 wk
WM39 VGP V600E HOM WT WT WT R24C CTNNB1, DCC, ERBB3, EZH1, GRM3, MET, SMARC4 RAC2 SOX10 CCND1 FGF3 FGF4 1:6 <1 wk
WM3912 MET N581Y WT WT WT L213F CDK4, CDK6, DDX3X, ERBB4, GRIN2A, GRM3, MAP3K9, NF1, ROS1, TERT CDNK2A CDKN2B   1:3 1 wk after established
WM3918 MET WT ND WT WT WT DCC, GRIN2A, PDGFRA, PTPRD, SOX10, TERT CDKN2A NF1    
WM3928 LN MET WT Y177X WT WT WT CDKN2A, CTNNB1, FGFR3, PTEN, TERT CDKN2B MET  
WM4002 MET V600E Hem Del WT WT WT MYC CDNK2A CDKN2B   1:6 3 days
WM46 MET V600E Hem Del WT WT R24C AKT3, STK11 CDNK2A CDKN2B FGFR3   1:10 <1 wk
WM47 MET V600E WT/HOM DEL WT WT WT NONE     1:6 1 wk
WM51 MET V600E ND WT WT WT AKT3 JAK3 KDR TP53     1:3 1 WK
WM75 VGP V600E ND WT WT WT EGFR, ERBB4, NF1, PREX2, TERT TRAPP     1:6 2-3 wks
WM8 MET WT WT WT N818K WT       1:3 1.5 wk after established
WM852 MET WT Hem Del Q61R HOM WT WT TP53, EZH2 CDNK2A CDKN2B   1:5 1 wk
WM853-2 VGP V600E ND WT WT WT CDKN2A, TERT TP53 MITF 1:6 1 wk
WM858 LN MET V600E ND WT WT WT GRM3, TERT CDKN2A TP53   1:6 1 wk
WM873-1 LN MET V600E ND WT WT WT GRM3, TERT CDKN2A TP53   1:6 2 wk
WM873-2 MET   ND WT WT WT       1:6 1 wk
WM88 MET V600E WT WT WT WT ALK, DCC, EGFR, MAP3K5, NF1, PREX2, PTPRD, SMARC4, TERT CDNK2A CDKN2B   1:5 4 days
WM9 MET V600E Hem Del/hom del WT WT WT GRIN2A, GRM3, PREX2, RAC1, RET, SMARCA4, TERT     1:5 1-2 wk
WM902B VGP V600E ND WT WT R24L AKT1, TERT   MITF 1:6 1 wk
WM983A VGP V600E WT WT WT WT TP53, TERT CCND2 MMP8 STK24 MITF  
WM983B MET V600E WT WT WT WT TP53, CDKN2A CCND2 MMP8 MITF 1:10 <1wk
WM983B BR BRAF Resistant V600E   WT WT WT TP53, CDKN2A, TERT CCND1 CCND2 FGF4 HRAS MMP8    
WM983C MET V600E WT WT WT WT CDKN2A, TERT, TP53 CCND2 CDKN2A MMP8 PTPRD STK24 MITF 1:6 1 wk
WM989 UNKNOWN V600E ND WT WT WT CDKN2A, PIK3CA, TP53, ROS1, EZH2, TERT CDNK2A CDKN2B PTPRD MITF FGF3 1:10 <1 wk