Molecular Screening and Protein Expression Facility

Overview

The Molecular Screening and Protein Expression Shared Resource fosters collaboration by providing expertise in biochemical and cell-based assay development for high-throughput screening and compound profiling.  Such assays enable researchers to identify small molecule compounds which interact with a target protein of interest.  These compounds can then be used as tools to further study the target proteins function and signaling pathways in cells.  The facility maintains a small molecule library of ~100,000 drug-like small molecules for high-throughput screening and provides access to liquid handling robotics and a multilabel plate reader.  In addition, the facility also provides access to Biacore T200 SPR instrument for label-free binding kinetics and a high-content screening system. Guidance is provided for target justification, assay development, miniaturization, robotic automation, and adaptation to HTS-compatible, high-density microplate (384-well) formats.  The facility provides production of recombinant proteins in E Coli, insect cells and mammalian cell systems and affinity purification of recombinant proteins upon request.  The facility also maintains glycerol stocks of shRNA libraries, which can be accessed by investigators for use in target validation studies. 

Services

Screening:

  • Development and optimization of miniaturized biochemical, cell, high-content, and label free SPR assays
  • High-throughput screening and pharmacological profiling of small molecule libraries
  • Data analysis and interpretation
  • Hit validation and quantitative assessment of compound activity required for post-screen advancement
  • Consultation, training, project management, and grant preparation

Protein:

  • Recombinant protein expression in bacteria, insect cells, or mammalian cells
  • Affinity purification of recombinant proteins
  • Distribution of shRNA library plasmids

Equipment & Features

  • Janus Verispan 8-tip automated pipetting workstation
  • Janus MDT automated pipetting workstation
  • NanoHead MDT dispense tool (0.05-0.5 ul)
  • EnVision multi-label microplate reader
  • PerkinElmer Operetta High-Content Screening reader
  • Biacore T200
  • MicroFlo bulk reagent dispensers
  • Amersham digital Western Blot system
  • Libraries of compounds spanning various pathways and targets – contact the facility for more information
  • BSL-1 and BSL-2 tissue culture suites

This facility is supported in part by a Cancer Center Support Grant (CCSG) awarded by the National Cancer Institute (NCI) to The Wistar Institute Cancer Center.

The facility was developed with support from the Commonwealth of Pennsylvania Department of Community and Economic Development Keystone Innovation Zone initiative, The F. M. Kirby Foundation, The CLAWS Foundation, The Florence & Daniel Green Foundation, The McClean Contributionship, From The Heart Foundation, the Noreen O’Neill Foundation for Melanoma Research, and NIH shared instrumentation grants.

Contact Us

The Wistar Institute 
Molecular Screening and Protein Expression Facility
Rooms 322 and 352
3601 Spruce Street
Philadelphia, PA 19104

Email: molecular_screening@wistar.org
Phone:
Molecular Screening: 215-495-3948
Protein Expression: 215-898-3814
Facility Hours: 
9:00 a.m.-5:00 p.m., Monday-Friday


Joseph Salvino, Ph.D.

Scientific Director

Joel Cassel
Managing Director

Barbara Niedziolka
Research Assistant III

Partnership with The Gene Editing Institute at Christiana Care

For inquiries or pricing related to gene editing, contact Dr. Eric Kmiec and the Gene Editing Institute team at geneeditinginstitute@christianacare.org or by phone at 302-623-0628.

Gene Editing Institute Partnership

The Gene Editing Institute at Christiana Care’s Helen F. Graham Cancer Center has partnered with The Wistar Institute.... Learn More >